Answer / falguni patel

Dead volume: It comprises the volume of HPLC system between
the point of injection to the point of detection, excluding
the part of column that contains packing.

Dwell volume: It includes the volume between pump and
column. in low pressure gradient it may be 1 mL & in high
pressure gradient it is to less.

Answer / aks

Dead Volume:Volume between injector to detector excluding
column volume (and also called as extra column volume)
Dwell Volume:Volume form the point of mixture to the column
entry (Also called as system delay volume)

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