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If we are getting split peak in Chromatogram,what we can do?
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Answer / alpesh patel
It means yr column is contaminated & the column needs
proper washing with suitable solvents. You can also
regenarate the column by proper washing with
polar solvent -> semi polar solvent -> non polar solvent ->
semi polar solvent -> polar solvent.
| Is This Answer Correct ? | 18 Yes | 4 No |
Answer / mr. bujji reddy kanchi
The peak split involves both mobile phase and stationery
phase. The pH of the mobile phase accuracy depends.
Mainly Stationery phase deterioration. Column has to be
regenerated according to the Column certificate
recommondation solvents.
| Is This Answer Correct ? | 6 Yes | 0 No |
Answer / satyasri
if u observe peak splitting,first u go to change(reduce) injection volume(means volume over load)...dn u go for buffer srength(means conc)...dn u go for pH of the buffer...dn only u go for checking of column performance(means whether u hv to check column was washed properly from previos one)........k
| Is This Answer Correct ? | 2 Yes | 1 No |
If the sample diluent is not compatable with mobile phase,
it could be the reason for peak splitting. Using mobile
phase as sample diluent would resolve the issue.
| Is This Answer Correct ? | 1 Yes | 0 No |
Main cause : That means u r column is contaminated
absorbant ion of impurites in the column.
Solution :
1)Wash calumn:-By polar solvent -> semi polar solvent -> non
polar solvent ->semi polar solvent -> polar solvent.
2)Check column performance
3)Replace column
| Is This Answer Correct ? | 0 Yes | 3 No |
Answer / umesh patel
BECOZ OF COLUMN OVERLOADING
DECREASE THE INJECTION VOLUME AND CHECK OUT
ALSO
IT HAPPENS THAT IMPURITIES FROM PREVIOUS INJECTIONS ELUTED
RUN BLANK WITH PROPER SOLVENT AND THEN WITH MOBILE PHASE
| Is This Answer Correct ? | 1 Yes | 5 No |
Answer / n.s.r
In Method development time or already validated method first we conform that already validated method it's ok but in development time we go on peak purity and finalise column problem or if any impurities is present in main peak.Thank you ANY WAY YOU WILL answer that question.
| Is This Answer Correct ? | 0 Yes | 6 No |
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