If we are getting split peak in Chromatogram,what we can do?
- 7 Answers
- 16295 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / alpesh patel
It means yr column is contaminated & the column needs
proper washing with suitable solvents. You can also
regenarate the column by proper washing with
polar solvent -> semi polar solvent -> non polar solvent ->
semi polar solvent -> polar solvent.
| Is This Answer Correct ? | 18 Yes | 4 No |
Answer / mr. bujji reddy kanchi
The peak split involves both mobile phase and stationery
phase. The pH of the mobile phase accuracy depends.
Mainly Stationery phase deterioration. Column has to be
regenerated according to the Column certificate
recommondation solvents.
| Is This Answer Correct ? | 6 Yes | 0 No |
Answer / satyasri
if u observe peak splitting,first u go to change(reduce) injection volume(means volume over load)...dn u go for buffer srength(means conc)...dn u go for pH of the buffer...dn only u go for checking of column performance(means whether u hv to check column was washed properly from previos one)........k
| Is This Answer Correct ? | 2 Yes | 1 No |
If the sample diluent is not compatable with mobile phase,
it could be the reason for peak splitting. Using mobile
phase as sample diluent would resolve the issue.
| Is This Answer Correct ? | 1 Yes | 0 No |
Main cause : That means u r column is contaminated
absorbant ion of impurites in the column.
Solution :
1)Wash calumn:-By polar solvent -> semi polar solvent -> non
polar solvent ->semi polar solvent -> polar solvent.
2)Check column performance
3)Replace column
| Is This Answer Correct ? | 0 Yes | 3 No |
Answer / umesh patel
BECOZ OF COLUMN OVERLOADING
DECREASE THE INJECTION VOLUME AND CHECK OUT
ALSO
IT HAPPENS THAT IMPURITIES FROM PREVIOUS INJECTIONS ELUTED
RUN BLANK WITH PROPER SOLVENT AND THEN WITH MOBILE PHASE
| Is This Answer Correct ? | 1 Yes | 5 No |
Answer / n.s.r
In Method development time or already validated method first we conform that already validated method it's ok but in development time we go on peak purity and finalise column problem or if any impurities is present in main peak.Thank you ANY WAY YOU WILL answer that question.
| Is This Answer Correct ? | 0 Yes | 6 No |
what is rs test why we are perfoming rs test
what is rt and rrt? how we find rrt? relation between rt and rrt/
why KBr is used in FTIR SPECTROSCOPY and why is it necessary to triturate KBr during FTIR analysis?
25 Answers Hikal, Lupin, Wockhardt,
What is cut off value of solvent used in hplc
what is the cleaning validation?
k.f principle in finding out moisture content
if instrument calibration fails then what require to do?
1)why use benzophenon,uracil in hplc calibration. 2)why use sucrose in polarimeter calibration. 3)what is S/N ratio, noise and drift in hplc 4)how we can find lod and loq in method validation
How to check hplc detecter sensitivity?
what is the significant of pH in HPlc?
difference between quality and quantity
How can we confirm the HPLC column is end-capped or not? Is it possible to identify by physical appearance?
Organic Chemistry 
