how to correct peak when peak comes broader in shape?
How to do calibration of HPLC?

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how to correct peak when peak comes broader in shape? How to do calibration of HPLC? ..

Answer / satish

if peak is broad then use dilute solution of triethylamine to sharpen the peak

Is This Answer Correct ?    5 Yes 1 No

how to correct peak when peak comes broader in shape? How to do calibration of HPLC? ..

Answer / chanti1984

1. ENHANCE THE COLUMN OVEN TEMPARATURE, REDUCE THE SAMPLE
LOAD N COLUMN AND CHANGE THE DILUENT
2. URACIL/CAFFIENE SOLUTION ARE USING FOR CALIBRATION OF
HPLC WITH THEIR RESPECTIVE WAVE LENGTH

Is This Answer Correct ?    1 Yes 2 No

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