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how to correct peak when peak comes broader in shape?
How to do calibration of HPLC?

Answers were Sorted based on User's Feedback



how to correct peak when peak comes broader in shape? How to do calibration of HPLC? ..

Answer / satish

if peak is broad then use dilute solution of triethylamine to sharpen the peak

Is This Answer Correct ?    5 Yes 1 No

how to correct peak when peak comes broader in shape? How to do calibration of HPLC? ..

Answer / chanti1984

1. ENHANCE THE COLUMN OVEN TEMPARATURE, REDUCE THE SAMPLE
LOAD N COLUMN AND CHANGE THE DILUENT
2. URACIL/CAFFIENE SOLUTION ARE USING FOR CALIBRATION OF
HPLC WITH THEIR RESPECTIVE WAVE LENGTH

Is This Answer Correct ?    1 Yes 2 No

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