Answer / balaji

Yes column dependant.

Why because:Total volume of mobile phase in a fully wet
packed column- the space between the particles of the
stationary phase plus the volume within the particles

So,based on length,internal diameter and particle size of
column it varies.

Optical Rotation*0.9477*100*200] Assay Of dextrose= ________________________________ 99.8* 200 why Factor=0.9477

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