If we are getting split peak in Chromatogram,what we can do?
Answer Posted / g.balaji
Main cause : That means u r column is contaminated
absorbant ion of impurites in the column.
Solution :
1)Wash calumn:-By polar solvent -> semi polar solvent -> non
polar solvent ->semi polar solvent -> polar solvent.
2)Check column performance
3)Replace column
| Is This Answer Correct ? | 0 Yes | 3 No |
Post New Answer View All Answers
Qc interview questions
How would you decide dissolution medium for NCE compound of class I drug
please explian me about area normalisation method,diluted standard method,impurity vs impurity and which one has to be selected in method development
Why only hydroxy naphthol blue indicator is used for standardisation of 0.05M EDTA solution instead of solochrome Black T or Euriochrome Black T indicator which is used for all sample analysis with 0.05M EDTA solution?
What is the difference between chromatographic purity and related substances?
WHY given much noise PDA dector then VWD Dector
cefoperozone and sulbactam inj. hplc test method
what is classification of elemental impurities? what is risk assement in elemental impurities?
In HPLC calibration, caffeine is used as primary standard for wave length calibration due to caffeine is having dual maxima at 273 & 205 nm and one minima at 245 nm. Any body can give reference of these details from any pharmacopeia (with chapter no.) or any other authentic guideline?
pharma industries oriented
What is delay volume?
What is the difference between purge septum flow and column flow in gas chromatography?
why we use Dichlorobenzene.nitrobezine.t-butyldi sulhate.for calibration of gc Head space
Which is the highly polar and highly non polar column in HPLC?
What is importance of pH in the Heavy metal test and How it effect on the test results?