Question { 18230 }

If we are getting split peak in Chromatogram,what we can do?

Answer

Main cause : That means u r column is contaminated
absorbant ion of impurites in the column.

Solution :
1)Wash calumn:-By polar solvent -> semi polar solvent -> non
polar solvent ->semi polar solvent -> polar solvent.
2)Check column performance
3)Replace column

Question { Dr Reddys, 35265 }

Why we are using only KBr in IR spectrometer why not KCl

Answer

KBr could not have any absorbance in IR range,but Kcl have
absorbance