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If we are getting split peak in Chromatogram,what we can do?

Question Posted / umesh patel

Answer Posted / umesh patel

BECOZ OF COLUMN OVERLOADING
DECREASE THE INJECTION VOLUME AND CHECK OUT

ALSO
IT HAPPENS THAT IMPURITIES FROM PREVIOUS INJECTIONS ELUTED

RUN BLANK WITH PROPER SOLVENT AND THEN WITH MOBILE PHASE

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