What is calibration procedure of gredient HPLC pump

Question Posted / bhavesh

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What is calibration procedure of gredient HPLC pump..

Answer / abhijeet gavali

You have two different methods for it
1.0 Use Propyl parabean in methanol and pure methanol
2.0 use 0.05% solution of acetone in water and water

Is This Answer Correct ?

14 Yes

3 No

What is calibration procedure of gredient HPLC pump..

Answer / drena

Mobile phase A is 5.6mg/l of propylparaben in methanol (port
A and B)
Mobile phase B is 100% methanol (port C and D)

Run the test for 20 min by using curve 11 method
sample is Methanol

Acceptance criteria:
A/B/C is between 9.5 % to 10.5
A/B/D is between 9.5 % to 10.5

Is This Answer Correct ?

13 Yes

5 No

What is calibration procedure of gredient HPLC pump..

Answer / kuchela rao athota

in waters gradient program

TIME PUMPA% pUMPB% PUMPC% PUMPD% CURVE
0.00 50% 50% 0 0
2.00 0% 0% 50 50 11
6.00 50% 50% 0% 0 11
10.00 45% 45% 10 0 11
12.00 50% 50% 0 0% 11
14.00 45% 45% 0 10 11
16.00 50% 50% 0 0 11
18.00 50% 50% 0 0 11

Is This Answer Correct ?

8 Yes

6 No

What is calibration procedure of gredient HPLC pump..

Answer / prerna

Gradient Programme for port A and C :
00 : 100
00 : 100
25 : 75
25 : 75
50 : 50
50 : 50
75 : 25
75 : 25
100 : 00
100 : 00

Above programme also run for port B and D

An increasing stepwise piramid will appear. Integrate that
peak and also split each step of the peak.

Find out its height and area of height

By using the formula ( which I don't remeber ), mean flow
of solvent at each step.
Flow : Limit
25 (24-26)
50 (49-51)
75 (74-76)
100 (100)

If the observed value is within the acceptance limit then
that pump passes the test.