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What is calibration procedure of gredient HPLC pump

Answer Posted / kuchela rao athota

in waters gradient program

TIME PUMPA% pUMPB% PUMPC% PUMPD% CURVE
0.00 50% 50% 0 0
2.00 0% 0% 50 50 11
6.00 50% 50% 0% 0 11
10.00 45% 45% 10 0 11
12.00 50% 50% 0 0% 11
14.00 45% 45% 0 10 11
16.00 50% 50% 0 0 11
18.00 50% 50% 0 0 11

Is This Answer Correct ?    8 Yes 6 No



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