Why we are performing wavelength accuracy at lower and
higher wavelengths in HPLC calibration
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Answer / tushar
this is because we can check absorbance in respective range
of minimum and maximum wavelength to get desire peak ,
retention time.
| Is This Answer Correct ? | 14 Yes | 3 No |
Answer / bhalchandra farde anuh pharma
We are perform wavelength accuracy test in lower and higher
wavelengthe b'coz of responce of compounds depend on the
wavelength max therefore it is necessary to ensure the
accuracy of the wavelegth set during analysis to produce
consistent result in both range.
| Is This Answer Correct ? | 4 Yes | 0 No |
Answer / d.thirupathi
The Uv detector range is 200nm to 400nm so that insted of
doing wave lenght accuracy at each level we are doing Lower
and higer wave lenghts so that it will cover all the uv
range.
| Is This Answer Correct ? | 5 Yes | 1 No |
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