Question { 24101 }

What is calibration procedure of gredient HPLC pump

Answer

Mobile phase A is 5.6mg/l of propylparaben in methanol (port
A and B)
Mobile phase B is 100% methanol (port C and D)

Run the test for 20 min by using curve 11 method
sample is Methanol

Acceptance criteria:
A/B/C is between 9.5 % to 10.5
A/B/D is between 9.5 % to 10.5

Question { Glochem, 50959 }

how to hplc calibrated

Answer

HPLC is calibrated by the following parameters:
1) Pump calibration
2) Detector Linearity
3)Injector Precision and carryover test
4) Wavelength accuracy by using caffeine and pyrene solution
5) Determine the column oven temp. and sample compartment temp
6) Gradient test
7) Detector noise

Question { Pharmaffiliates Analytics and Synthetics, 16944 }

What is the thickness of polystyrene film?

Answer

0.35 µm in thickness