how can you do the analysis of compound given to u by hplc
Answers were Sorted based on User's Feedback
Answer / guru
*first need to check pH .If it is acidic we will go for ODS column.
* If it is basic we will go for BDS.
|Is This Answer Correct ?||0 Yes||0 No|
Answer / rajesh sadhwani
IN POLARIMETRY WE PASS POLY RADIATION TO THE NICOL PRISM
THEN IT CONVERTS TO MONOCROMATIC,IT IS HAVING NO DIRECTION
AND ONLY ONE FREQUENCY, WHEN THAT PASS THORUGH THE CHIRAL
CENTER THEN ONLY ROTATE.
WITHOUT PRESENCE OF LIGHT IT DO NOT ROTATE
THINK MACRO LEVEL. MOLECULES OF SUBSTANCE WITH CHIRAL
CENTRE ARE ALLIGNED IN SUCH A WAY SO THERE SHOULD BE LESS
REPULSION. THINK OF PUSH OF LIGHT PASSES BY. AGAIN THINK OF
GROUPS AND ATOMS CONNCTED TO CHIRAL CENTRE. IN THIS
SITUATION LIGHT WAVES WILL PASS ONLY IN ONE DIRECTION
BECUAS OF CLOSSEST ARRANGEMENT OF MOLECULES.
|Is This Answer Correct ?||0 Yes||4 No|
if the flow rate is increases then any changes in retention time in hplc
What is the purpose & importance of using the polystyrene film in ir calibration?
how v can identify functional groups by wet techniques?
what is defferance between dipolemoment and polarity
WHY KBr is used in IR Spectroscopy
various methos to calculate precision in validation methods in HPLC?
sop of a uv visible spectrophotometer double beam elico model
why we are used 4,7,9,2 buffers for the ph calibration
How you will select column for HPLC development depends on nature of compound and how it will work explain in brief?
What is the difference between HPLC and GC?
In UV calibration we use Holmium perchlorate solution made in 1.4M perchloric acid The question is that 1.4 M perchloric acid we make is it in water or glacial acetic acid?
plz tall me that any pharmacopeia and guideline is par meted to use 1.68,4.01,7.00,9.00,12.45 buffer for ph meter calibration