how can you do the analysis of compound given to u by hplc




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how can you do the analysis of compound given to u by hplc..

Answer / guru

*first need to check pH .If it is acidic we will go for ODS column.
* If it is basic we will go for BDS.

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how can you do the analysis of compound given to u by hplc..

Answer / rajesh sadhwani

IN POLARIMETRY WE PASS POLY RADIATION TO THE NICOL PRISM
THEN IT CONVERTS TO MONOCROMATIC,IT IS HAVING NO DIRECTION
AND ONLY ONE FREQUENCY, WHEN THAT PASS THORUGH THE CHIRAL
CENTER THEN ONLY ROTATE.
WITHOUT PRESENCE OF LIGHT IT DO NOT ROTATE

THINK MACRO LEVEL. MOLECULES OF SUBSTANCE WITH CHIRAL
CENTRE ARE ALLIGNED IN SUCH A WAY SO THERE SHOULD BE LESS
REPULSION. THINK OF PUSH OF LIGHT PASSES BY. AGAIN THINK OF
GROUPS AND ATOMS CONNCTED TO CHIRAL CENTRE. IN THIS
SITUATION LIGHT WAVES WILL PASS ONLY IN ONE DIRECTION
BECUAS OF CLOSSEST ARRANGEMENT OF MOLECULES.

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