how to regulate noise in HPLC?
Answers were Sorted based on User's Feedback
Answer / santosh
Don't select wavelength nearer to cutoff of mobile phase or prepare mobile phase transpersonal to selected wavelength
| Is This Answer Correct ? | 3 Yes | 0 No |
Answer / a.iyyappan
Baseline noise can be regulated by using HPLC grade solvents and high purity buffer salts.
Detector wavelength should be follow UV CUTT OFF POINT.
| Is This Answer Correct ? | 2 Yes | 0 No |
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hii, i'm had a sample of 1L that gone through extraction & evaporation. i used 0.5ml of methanol for reconstitution before injecting the sample to HPLC. i made a calibration curve using a standard solution but now i'm confused about how to display the concentration i got from the sample. for ex. i got 0.2ug/ml how i'm gonna explane this is for the 1L ? please help .. i'll really appreciate it thanks
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