Why we use Uracil for HPLC calibration in Detector wave
ength accuracy test?
Answers were Sorted based on User's Feedback
Answer / poojitha
uracil is a primary standard and shows maximum absorbance
at 260nm
| Is This Answer Correct ? | 30 Yes | 9 No |
Answer / dhayasiva
Uracil is used as void volume indicator, Hence it is not
retained in HPLC column
| Is This Answer Correct ? | 15 Yes | 7 No |
Answer / gangi reddy
Since it is a standard, Its a unretained substance in the
HPLC Column. No column is required for its elution.
| Is This Answer Correct ? | 4 Yes | 4 No |
Answer / mr. bujji reddy kanchi
Since it is a standard, Its a unretained substance in the
HPLC Column. No column is required for its elution.
| Is This Answer Correct ? | 5 Yes | 8 No |
Answer / flamingo rabale adl
1.What is stability indicating assay/ RS method ?
2. What is descriminatory disolution medium ?
| Is This Answer Correct ? | 6 Yes | 23 No |
How to select buffer for HPLC mobile phase
4 Answers Cadila, Cipla, Lupin,
how pda detector works over uv?
what is lod and loq ?,why use k2cr2o7 , kcl h2so4 in uv calibration ?,why use benzophenone & caffene acetone in hplc calibration ?,what is leading peak in hplc ?why we do the calibration of limit of stry light in hplc & uv ?
which lights using during force degradation?
give clarity of linearity and range in method validation
How to decide the standard concentration for related substances hplc method
How we can identify process related and degradation impurity in single method with short period?
What is Spliit and Splitless in Gas Chromatography nad How does it work?
Explain about Low pressure gradient and High Pressure gradient systems in HPLC?
4 Answers Mylan, Orchid, Roshanico,
How they found 1mL of K.F reagent is equivalent to 5mg of water and if we change the composition of K.F reagent, is it can neutralize more amount of water?
what is the principal of malvern particle sizer & its used in pharma industry
principle of AAS & its uses ?