How to check hplc detecter sensitivity?
Answer / babasaheb gilbile
In Waters HPLC 1 ml flow for methanol passing with wavelenth 230nm. and after 1hr check the Detector lamda max value. If ie greater than 20 the detector sensitivity is good
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2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.
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Colorimtry