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tell me about aliphatic acid estimation by hplc which columns can we use

tell me about aliphatic acid estimation by hplc which columns can we use..

Answer / reddy

A colorimetric method for the determination of carboxylic acids based on the dicyclohexylcarbodiimide-coupled reaction of 2-nitrophenylhydrazine and carboxylic acids is described. The product of the reaction is extracted into aqueous sodium hydroxide to produce a blue color. This method is suitable for the analysis of aliphatic acids, but aromatic acids do not react under these conditions.

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