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Analytical Chemistry Interview Questions
Questions Answers Views Company eMail

What are the different types of columns used in HPLC


3 10164

In HPLC when we use Isocratic method, Gradient method in hplc?


8 17226

Any molecule is filing for ANDA purpose, what are the dissolution requirements / specifications needed ?


1 4631

What is Endcapped Hplc column

2 7653

Why we more noise in PDA detector compared to UV detector?


1 9640

if the purity is 99.9%,is need to do assay?

Reddy Labs,

3 12528

What is the difference between specified impurity and Identified impurity


3 12462

What is the difference between specified impurity and Identified impurity

Merchant Navy,

1 3839

how do you established the RRF for unknown impurity

Square Pharma,

4 9076

I want analyze my compound at 325 nm so which standard shall i use to calibrate my HPLC system? How i can assure my hplc detector is suitable or not?


3 7375

How we will choose pH of a mobile phase if analyte pKa value is 4

Celon Labs, Laurus,

2 9216

what parameters we will consider while developing a HPLC method and how we confirm our HPLC method is valid?

DRL, Laurus,


Which column is more polar, C8 or C18?

Cipla, Glenmark, Laurus, Torrent Power,

6 24439

what is the difference between heavy metals and sulphated ash


2 14612

what is difirence b/w ROI&ROE


1 7549

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Un-Answered Questions { Analytical Chemistry }

why does used dry caffien in HPLC calibration?


How to choose the which salt is suitable for mobilephase


2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.


What is the principle of Thermal conductivity detector and FID?


How do we fix the sample concentaryion in hplc method development. What is the basis?


For standardization of volumetric solutions, acceptance limit for concentration is considered as 10% of nominal concentration and %RSD as NMT 0.2% (for appropriate no. of replicates). But , if %RSD has not met, what should be further action?


How to start the dissolution development for unknown tab?


Why do we use KMnO4 in the test of control of obsorbance ? and why do we take specific quantity i.e 57-63mg?


What is similarity factor and its use in api strength?


what is peak purity and its formula


WHY given much noise PDA dector then VWD Dector


Why dissolution test is not performed in all of the products


In BP2013, Loperamid HCl monograph. Assay by titration with 0.1N sodium hydroxide using hydrocloric acid 0.01N and reading the volume added between the 2 points of inflexion. I have a question that if the diluent solvent is ethanol is certainly consumed a amount of volume of titrant, so this volume must be eliminated on the result calculation or not apart from first point which is subtracted above.


Loss of linearity in tkn analysis, possible reasons?


In GC calibration,what is the procedure to calibrate the TCD (thermal counductivity detector)suggest ?