what is a differance between assay by HPLC and assay by
titration?
- 5 Answers
- 28676 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / venkat
In Titration Analysis imppurities also reacting with
titrant because impuirties having the simlar strature of
main analyte but in HPLC Analysis all impurities are
saparated from main Analyte(Specific)
| Is This Answer Correct ? | 29 Yes | 1 No |
Answer / sridhar
some of the compound are not response in uv-vis region in
this case go for titration for determine the assay in product
| Is This Answer Correct ? | 19 Yes | 1 No |
Answer / supriya
1. Some of the compounds only detected in manual titrations based on colour changed.
2.some of the compounds are detected by electrode by autotitrator.
3.Some of the compounds(we should not able to identifie the end point by adding indicator) will respond only Based on the nanometer we can analyse by Assay by HPLC these type of compounds are not responded by the indicators.
The electrode is also nothing but one type of indicator.The electrode will respond Based on the milli volts and pH,there is no need to color identification(mens end point).
| Is This Answer Correct ? | 2 Yes | 2 No |
Answer / kanakadurga
Assay by HPLC:
It explains how much of our analyte is(i.e Content of our
analyte).It is not related to purity of analyte.
For example:
Assume an analyte of x with 99.5% purity.And prepare
20%,50%and 85% solutions and inject in HPLC.
For 20% solution you will get 99.5% Purity and 20% Assay.
For 50% solution you will get 99.5% Purity and 50% Assay.
For 85% solution you will get 99.5% Purity and 85% Assay.
| Is This Answer Correct ? | 4 Yes | 20 No |
Answer / asha latha
Assay by Titration is qualitative anlysis where as Assay by
HPLC is a quantitative analysis
| Is This Answer Correct ? | 4 Yes | 38 No |
What is similarity factor and its use in api strength?
Why we compare unequal concentrated solutions in heavy metals test. For example comparing the test solution with 1 ppm lead standard and telling it's less than 20ppm?
1 Answers Micro Labs, Mistair,
when we have polar type of sample and solvnt hen which type of stationry phase we have to use
Why do we use KCl ,K2Cr207,Toulene & Halonium oxide for the caliberation of UV
why buffer is added in solvent system in HPLC ??
process of Diclofenac sodium,IP.
why we calibrate ph meter and balance daily.....but hplc and uv requires quaterly?
why hexadecane is used in calibration of FID IN GC THERE IS NO REMAINING SOLVENTS
Why use the Omeprazole as a reference standard to estimate the assay of esomeprazole by HPLC method
How you will select the runtime in HPLC for a drug in method development.
6 Answers Aurobindo, GVK, Lesanto Laboratories,
Why 1.2% KCl is used for limit of stray light? and why limit of stray light is required in UV calibration?
what is the structure of DOWEX-50
Organic Chemistry 
