Answer / venkat

In Titration Analysis imppurities also reacting with
titrant because impuirties having the simlar strature of
main analyte but in HPLC Analysis all impurities are
saparated from main Analyte(Specific)

Answer / sridhar

some of the compound are not response in uv-vis region in
this case go for titration for determine the assay in product

Answer / supriya

1. Some of the compounds only detected in manual titrations based on colour changed.
2.some of the compounds are detected by electrode by autotitrator.
3.Some of the compounds(we should not able to identifie the end point by adding indicator) will respond only Based on the nanometer we can analyse by Assay by HPLC these type of compounds are not responded by the indicators.
The electrode is also nothing but one type of indicator.The electrode will respond Based on the milli volts and pH,there is no need to color identification(mens end point).

Answer / kanakadurga

Assay by HPLC:
It explains how much of our analyte is(i.e Content of our
analyte).It is not related to purity of analyte.
For example:
Assume an analyte of x with 99.5% purity.And prepare
20%,50%and 85% solutions and inject in HPLC.
For 20% solution you will get 99.5% Purity and 20% Assay.
For 50% solution you will get 99.5% Purity and 50% Assay.
For 85% solution you will get 99.5% Purity and 85% Assay.

Answer / asha latha

Assay by Titration is qualitative anlysis where as Assay by
HPLC is a quantitative analysis

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