Answer / ram kumar

Firstly the basic difference is no. of Carbon atoms. C-18 has higher retention and Hydrophobicity as compared to the C-8.

i set electrode to 7 buffer than i used it to check 4 buffer and 10 buffer if the results are not as expected than is it better to set the buffer again to 4 buffer or is there any other method available please let me know ...

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why using buffers in hplc inteed of water

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