in HPLC injection volume is increase ,R.T will increase ?

Answers were Sorted based on User's Feedback



in HPLC injection volume is increase ,R.T will increase ?..

Answer / b.gangatharan

In hplc area will increase when injection volume is increased.But rt will not increase or change.

Is This Answer Correct ?    27 Yes 1 No

in HPLC injection volume is increase ,R.T will increase ?..

Answer / srikar_22

Increasing sample volume did not show any affect on rt

Is This Answer Correct ?    5 Yes 1 No

in HPLC injection volume is increase ,R.T will increase ?..

Answer / ravi1234

There will be no relation Between Inj Volume and Retention time.If Injection Voulme Increases Area of Peak Changes..Not RT.

Is This Answer Correct ?    2 Yes 0 No

in HPLC injection volume is increase ,R.T will increase ?..

Answer / bindeshwar prasad

Whenever sample concentration will increases there is chance to slight decrease in Rt with respect to lower concentrations Rt.

Is This Answer Correct ?    2 Yes 2 No

in HPLC injection volume is increase ,R.T will increase ?..

Answer / sachin

because of more sample concentration peak will take time to elute.

Is This Answer Correct ?    7 Yes 18 No

Post New Answer

More Analytical Chemistry Interview Questions

What is the difference between chromatographic purity and related substances?

1 Answers  


Tell me something about Vitamin A test method by HPLC

1 Answers   Lupin,


What is split ratio in Gc? Splitless? how requirr to select?

1 Answers  


why disodium tartarate is used for autotitrator calibration

2 Answers   Cipla, Micro Labs,


what is regration method in method validation , how we can calculate it.

3 Answers   Cipla, Sun Pharma,


why we want incorporate area of each solution of Intermediate precision and repeatability as well as Accuracy ? What is the use to incorporate area of each solution of Intermediate precision and repeatability as well as Accuracy ?

1 Answers  


What is gelatinization?

1 Answers   Nestle,


How to interpret XRD graphs against standard and limit for same?

3 Answers   NCL,


What is similarity factor and its use in api strength?

1 Answers  


Why water not directed by FID detector 

2 Answers   Amoli Organics, Lupin,


why do we adjusted ph in the mobilphase

9 Answers   Cipla, Dr Reddys, Reddy Labs,


what is lod and loq

3 Answers  


Categories
  • Organic Chemistry Interview Questions Organic Chemistry (342)
  • Inorganic Chemistry Interview Questions Inorganic Chemistry (123)
  • Analytical Chemistry Interview Questions Analytical Chemistry (1387)
  • Physical Chemistry Interview Questions Physical Chemistry (62)
  • General Chemistry Interview Questions General Chemistry (478)
  • Chemistry AllOther Interview Questions Chemistry AllOther (190)