in HPLC injection volume is increase ,R.T will increase ?
Answer Posted / bindeshwar prasad
Whenever sample concentration will increases there is chance to slight decrease in Rt with respect to lower concentrations Rt.
Is This Answer Correct ? | 2 Yes | 2 No |
Post New Answer View All Answers
which situation gc hs and gc als require to use?
in which situation require to take incident in validation?
before starting analytical method valodation what you checking? and how giving preference to start validation?
WHICH STANDARD WE USE IN SILICA ANALYSIS IN SILICA-GEL METHOD FOR SOLID SAMPLE
how to set sample and standard concentration in RS method?
Difference between the quantitative analysis and qualitative analysis?
why we use Dichlorobenzene.nitrobezine.t-butyldi sulhate.for calibration of gc Head space
If suppose 10 methods of dissolution given in pharmacopoeial for single content product then which method out of 10? or all 10 require to follow?
Ratio of Water and Acetonitrile(diluent) is not recommended to use in cleaning method validation,why?
What is third generation HPLC Columns?
how you establish the LOQ?
Describe your field experience sample type collected,sample techniques,field measurements taken and equipment used?
How to set specification of assay, fisdolution and related substances?
what parameters we will consider while developing a HPLC method and how we confirm our HPLC method is valid?
Which type of column should i use to check the purity of high molecular weight protein using HPLC reverse phase column chromatography? Hi everyone. I wanna to check the purity of high molecular weight protein (collagen) with MW of ~130 kDa using a HPLC. I know C18