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Why we use Caffeine in hplc calibration
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Answer / padma
It gives a multiwavelength response with Maxima at 205nm and 273nm and minima at 245nm ,it is stable for long time and easily avialable in market and cheap.It is specially for wave length accuracy since it will scan the whole range of UV
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Answer / venkatesh
Caffeine is thermally stable natural product, Detects on
multi wavelengths like 273,254,etc.. and also not degradable.-
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Why we use caffeine for HPLC calibration in Detector wave ength accuracy test
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