Interested to Buy Any Domain ? << Click Here >> for more details...
How you will select column for HPLC development depends on
nature of compound and how it will work explain in brief?
- 3 Answers
- 10984 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / debananda mishra
Polar compounds can be analysed throgh polar stationary
phase and nonpolar compouds can separated through nonpolar
stationary phase but mobile phase used is vice versa.
| Is This Answer Correct ? | 4 Yes | 1 No |
Answer / muzammil thange
Compounds should be seperated on "like likes like basis" ie polar compounds should be separated on polar columns and vice versa. Polar compounds when analysed on C-18 should be treated with modifiers to act like non-polar or neutral compounds.
| Is This Answer Correct ? | 4 Yes | 2 No |
Answer / sarvesh pathak
1.if compound is polar select midpolar or towards the
nonpolar column e.g. C18 is midpolar column which is used.
2.if compound is nonpolar select polar stationary phase
e.g. silica column
3.look for the nature of compound whether it is ionisable
or non-ionisable
| Is This Answer Correct ? | 4 Yes | 6 No |
identification is for unknown? qualification for known? reporting for LOQ?
For limit test of heavy metals in BP, Method C require that the substance is ignited at a temperature not exceeding 800 °C. Why confines such the temperature?
Who discovered X-ray
2 Answers Huntsman Corporation,
Why pH range is maximum 14.0
how decide the clining method and cleaning method validation require for this perticular products?
why holium oxide prepared in 10% HCLO4 SOLUTION
A ha tc is actual defination of retention time?
how you start RS method development when for new product?
what is biorelivent dissolution media?
What is calibration procedure of gredient HPLC pump
What is reaction takes place when water content analysis is done
What is the difference between kf and LOD
Organic Chemistry 
