Interested to Buy Any Domain ? << Click Here >> for more details...
How you will select column for HPLC development depends on
nature of compound and how it will work explain in brief?
- 3 Answers
- 10931 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / debananda mishra
Polar compounds can be analysed throgh polar stationary
phase and nonpolar compouds can separated through nonpolar
stationary phase but mobile phase used is vice versa.
| Is This Answer Correct ? | 4 Yes | 1 No |
Answer / muzammil thange
Compounds should be seperated on "like likes like basis" ie polar compounds should be separated on polar columns and vice versa. Polar compounds when analysed on C-18 should be treated with modifiers to act like non-polar or neutral compounds.
| Is This Answer Correct ? | 4 Yes | 2 No |
Answer / sarvesh pathak
1.if compound is polar select midpolar or towards the
nonpolar column e.g. C18 is midpolar column which is used.
2.if compound is nonpolar select polar stationary phase
e.g. silica column
3.look for the nature of compound whether it is ionisable
or non-ionisable
| Is This Answer Correct ? | 4 Yes | 6 No |
When ever we perform system suitability,inject the corresponding standard 6 times why? is thair any reson
i want to know about headspace and interview question on headspace
1 Answers Srikem Laboratories,
Hi sir if any product monograph not given known impurities (may be 5 impurities) specifications then how we require to proceed for inhouse formulation?
In C8,C18....etc have 4&5 etc... microns Columns is there like that how to separate Chiral columns as per Micron size?
Why karl fisher factor is 5 ?
what is defferance between dipolemoment and polarity
Why only 1.2 million lux hours require in photostability study?
on saturation solubility study data how we can find out the bcs class of drug?
is it necessary to do HPLC calibration at wavelength 315nm if we are doing analysis at this wavelangth
If suppose 10 methods of dissolution given in pharmacopoeial for single content product then which method out of 10? or all 10 require to follow?
what is difference between UV - VISIBLE MODEL NO like 1600,1601,1700 etc ? plz explain me
0 Answers Graviti Pharmaceuticals,
why 0.02% solution toluene &noHexane using in uv calibration?
1 Answers Graviti Pharmaceuticals, Orbit Pharma, Ranbaxy, Vimta Labs,
Organic Chemistry 
