in what basis wavelength will select for a compound at
initial method development trials
Answers were Sorted based on User's Feedback
Answer / ramkrishna thakare
1 ) for any unknown compound firstly solubility check.
2 ) then on the basis of solubility UV - scanning should
be done
3 ) on the basis of UV - scanning Maxima must be
checked.& then on the basis MAXIMA wavelength should be
selected.it may multiwavelength or single-wavelength maxima .
RAMKRISHNA THAKARE -------SANDOZ
Is This Answer Correct ? | 29 Yes | 1 No |
Answer / u.venkatramireddy
Based on Chromophore in the compound
posted by uvreddy
DrReddy's
Is This Answer Correct ? | 4 Yes | 2 No |
Answer / vijay
Based on wavelength of the compound. Not only lambda max we have to consider co-elute peak, cut off value of solvent.
Is This Answer Correct ? | 1 Yes | 0 No |
Answer / shami thakur
Not mentioned type of method development UV or HPLC
During hplc method development mobile phase are selected according to dissociation constant value and all study carried with the help of PDA detector wavelength range 200-400nm
Is This Answer Correct ? | 1 Yes | 0 No |
importance of internal standard and in what conditions it is used?
Why we are having specification 100% above in case of assay as there is only 100% of compound?
2 Answers MSN Pharma, Nectar Lifesciences,
what is mean by covalidation
Please give idea about method development for cleaning method and how maco level establish
which are the sizes of capsules?
when we have polar type of sample and solvnt hen which type of stationry phase we have to use
How we develop a method by HPLC for unknown compound,if we got three peaks with same response then how we can find out which one is our desired compound's peak without taking help of LC MS.
what is method development parameter in HPLC method development
In Analytical method development related substance method when did follow w/w method when did follow area normalisation method
2 Answers Dr Reddys, Sun Pharma,
How to make up 100ml of a solution cintaining compound A at 95µg/ml and compound B at30µg/ml for an HPLC reference standard.
What is FFA, PV, and PAV value in oil.
how can you determine a compound acidic or basic?