in what basis wavelength will select for a compound at initial method developm

in what basis wavelength will select for a compound at
initial method development trials

Question Posted / kishan05

4 Answers
12790 Views
Matrix, I also Faced
E-Mail Answers

Answers were Sorted based on User's Feedback

in what basis wavelength will select for a compound at initial method development trials..

Answer / ramkrishna thakare

1 ) for any unknown compound firstly solubility check.
2 ) then on the basis of solubility UV - scanning should
be done

3 ) on the basis of UV - scanning Maxima must be
checked.& then on the basis MAXIMA wavelength should be
selected.it may multiwavelength or single-wavelength maxima .

RAMKRISHNA THAKARE -------SANDOZ

Is This Answer Correct ?

29 Yes

1 No

in what basis wavelength will select for a compound at initial method development trials..

Answer / u.venkatramireddy

Based on Chromophore in the compound

posted by uvreddy
DrReddy's

Is This Answer Correct ?

4 Yes

2 No

in what basis wavelength will select for a compound at initial method development trials..

Answer / vijay

Based on wavelength of the compound. Not only lambda max we have to consider co-elute peak, cut off value of solvent.

Is This Answer Correct ?

1 Yes

0 No

in what basis wavelength will select for a compound at initial method development trials..

Answer / shami thakur

Not mentioned type of method development UV or HPLC
During hplc method development mobile phase are selected according to dissociation constant value and all study carried with the help of PDA detector wavelength range 200-400nm

Is This Answer Correct ?

1 Yes

0 No

Post New Answer

More Analytical Chemistry Interview Questions

What should be the calibration procedure for wavelength accuracy in case of HPLC?

4 Answers

how would you determine the acceptance criteria for cleaning validationof product A for product B?

1 Answers Cipla, Cypher Solutions, Torrent Power,

Why we are using only KBr in IR spectrometer why not KCl

10 Answers Dr Reddys,

why phenolpthalin gives pink colour when we titrate aganist sodium hydroxcide & phenolpthalin gives colourless titrate aganist hydrochlric acid which is un burate

1 Answers

How we can analysed unknown sample by hplc....

2 Answers Archimedes,

perchloric acid&glacal acetic acid both are acids but reaction is complete in volumetric titrations.how?

1 Answers

what is definition of validation? which components are followed give detail?

0 Answers

IS THERE ANY EQUIPMENT TO CHECK AND CALCULATE THE POLARITY OF A LIQUID?

0 Answers Reddy Labs,

why water, methanol, ethanol ets solvents are used in UV SPECTRIPHOTOMETER for blank reading ?

7 Answers

Why only 1.2 million lux hours require in photostability study?