What is the reason of RT change in new column v/s old column in HPLC

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What is the reason of RT change in new column v/s old&n..

Answer / naresh

Regular usage of old column some stationary phase is loss inside the column compared to new column so compound retention inside the column varies between these two columns

Is This Answer Correct ?    7 Yes 0 No

What is the reason of RT change in new column v/s old&n..

Answer / arul

old coloumn inside some particles may be formed, new coloumn no particles that is reason

Is This Answer Correct ?    2 Yes 0 No

What is the reason of RT change in new column v/s old&n..

Answer / dipty

Because the old column might not be washed properly so the stationary phase which is not used up that is if the column is new it has more chance to hold up the drug.

Is This Answer Correct ?    3 Yes 3 No

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