What is the reason of RT change in new column v/s old column in HPLC
Answers were Sorted based on User's Feedback
Answer / naresh
Regular usage of old column some stationary phase is loss inside the column compared to new column so compound retention inside the column varies between these two columns
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Answer / arul
old coloumn inside some particles may be formed, new coloumn no particles that is reason
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Answer / dipty
Because the old column might not be washed properly so the stationary phase which is not used up that is if the column is new it has more chance to hold up the drug.
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