Golgappa.net | Golgappa.org | BagIndia.net | BodyIndia.Com | CabIndia.net | CarsBikes.net | CarsBikes.org | CashIndia.net | ConsumerIndia.net | CookingIndia.net | DataIndia.net | DealIndia.net | EmailIndia.net | FirstTablet.com | FirstTourist.com | ForsaleIndia.net | IndiaBody.Com | IndiaCab.net | IndiaCash.net | IndiaModel.net | KidForum.net | OfficeIndia.net | PaysIndia.com | RestaurantIndia.net | RestaurantsIndia.net | SaleForum.net | SellForum.net | SoldIndia.com | StarIndia.net | TomatoCab.com | TomatoCabs.com | TownIndia.com
Interested to Buy Any Domain ? << Click Here >> for more details...


why buffer is added in solvent system in HPLC ??

Answers were Sorted based on User's Feedback



why buffer is added in solvent system in HPLC ??..

Answer / balu

I thing to maintain the pH of solution

Is This Answer Correct ?    30 Yes 2 No

why buffer is added in solvent system in HPLC ??..

Answer / t reddy

buffer is used to keep PH stable in ionizable condition.

Is This Answer Correct ?    6 Yes 0 No

why buffer is added in solvent system in HPLC ??..

Answer / haribabu

in Reverse phase chromatography for good resolution

Is This Answer Correct ?    5 Yes 2 No

why buffer is added in solvent system in HPLC ??..

Answer / msreddyvelagala

in solute we may have different products,all these cannot elute at same concetration of mobilephase in short time.solvent is polar,to get the eluates faster we have tochange the mobilephase composition(i.e polarity to be increased up to certain time intervel)

Is This Answer Correct ?    3 Yes 1 No

why buffer is added in solvent system in HPLC ??..

Answer / sasibhushanreddy

to control the ph of the soultion

Is This Answer Correct ?    4 Yes 3 No

why buffer is added in solvent system in HPLC ??..

Answer / vikasnautiyal4

because solvent system intrect
with mixture (they are mixture
of acid and base) to prevent
tha ionisation and controle
ph of analyte.

Is This Answer Correct ?    0 Yes 0 No

why buffer is added in solvent system in HPLC ??..

Answer / vijay bhosale

To maintain the PH oo solution.

Is This Answer Correct ?    0 Yes 0 No

why buffer is added in solvent system in HPLC ??..

Answer / manisha dawale

To prevent ionization,to control retension and for proper peak shape

Is This Answer Correct ?    0 Yes 0 No

why buffer is added in solvent system in HPLC ??..

Answer / tapan shaw

To simulate the dissolution behavior in our body fluid
because our body fluid where drugs release occur is either
acidic or basic.

Is This Answer Correct ?    1 Yes 3 No

why buffer is added in solvent system in HPLC ??..

Answer / ankita

to prevent ionization of weak acids and weak bases.

Is This Answer Correct ?    2 Yes 6 No

Post New Answer

More Analytical Chemistry Interview Questions

what is PH of 0.1N Hcl, 0.01N Hcl & Mehanol.

3 Answers  


what is mean by theoritical plates(not formula) ?

4 Answers   Micro Labs,


why we make titiration of Iron with EDTA ?

1 Answers   Cipla, Sitec Labs,


why we use only vanilline , acetanilide,& caffeine For the Calibration of melting point appartus?

7 Answers   Reddy Labs,


What is the difference between c8 and c18 hplc column?

1 Answers  


how std. of znso4

1 Answers  


Why pyrene is also use for calibration if detector

1 Answers   Cipla, Dr Reddys,


If we do accuracy at same concentration at which linearity planned,what is the need to do linearity separately?

0 Answers  


what is difference between drug products and drug substance?

5 Answers   DRL,


how to calculate the Step and ripple accuracy for pump gradient test for UPLC?

1 Answers   Wockhardt,


what are the primary standards of redox titrations?Name any two.

2 Answers   Laurus,


Near infrared Principle

1 Answers  


Categories
  • Organic Chemistry Interview Questions Organic Chemistry (342)
  • Inorganic Chemistry Interview Questions Inorganic Chemistry (123)
  • Analytical Chemistry Interview Questions Analytical Chemistry (1387)
  • Physical Chemistry Interview Questions Physical Chemistry (62)
  • General Chemistry Interview Questions General Chemistry (478)
  • Chemistry AllOther Interview Questions Chemistry AllOther (190)