Answer / shyam kumar

Capillary columns 100 micron or less in internal diameter
so the sample vlume what we are injected is too high for
column thus split help to dived the volume and pass require
volume into injuctor.
Eg : Split ratio is 1 : 10 that means what ever the sample
u injected that portion is divided into 10 parts from that
10 parts only 1 part will go into injuctor. this is to
minimize the load.

Answer / sures babu

Capillary columns 100 micron or less in internal diameter
so the sample vlume what we are injected is to high for
column thus split help to dived the volume and pass throuh
less amount and remaning sample gone as a wasre.

Answer / daljit singh randhawa

Split ratio:- It is the ratio of sample amount inject into
the column to that waste out from glass insert.
In capillary columns the size of bore is about 5 microns.It
faced xtra load whent inject high conc. sample.for reduce
the xtra load on column there is a need of split ratio.
Normaly its value:- 10:1
split ratio is required when Conc.of sample >or=0.1 %
split ratio is not required when Conc.of sample < 0.01 %

Answer / jajili eluru

Split injection techniques are used in capillary gas
chromatography. Capillary columns are easily overloaded, so
smaller amounts have to be injected. Because of the split a
smaller amount of analyte will enter the column



Read more:
http://wiki.answers.com/Q/What_is_use_of_split_ratio_in_gc_a
nalysis#ixzz1O1XZTnZM

Answer / khaja mohinuddin

Capillary columns are easily overloaded by injecting High Amount of Sample Concentration.
To Reduce or overcome this problem Split Ratio is Incorporated The Main use of this Split Ratio is Inject Less amount of Sample of interest into column or passes through it.
The Q? comes in a mind How it could be Done
Split vent is option Provide by Every manufacture like Agilent and shimadzu how it works i will expalin
Example :- ur method shows a split ratio of
1:30 Metan that 150ml/min of Split vent flow and Colum flow 5ml/min If u have Installed 0.53mm id Colum of any pahse.
Similarlly 1:50 mean 250ml/min of split ventflow and 5ml/min of column flow

formula split ratio = split vent flow / column flow
if know any of the parameter u can calculate by substituting in the above formula ...


thanks

Answer / vijay more

Split ratio is split of flow and thus split of sample at
the injection port to minimize sample overload to GC
capillary column.

Answer / rohith

capilarry columns are easily overloaded by injection of high volume of analyres to minimize this we use spit4 dat samples

Answer / piyush

I am using gc now days when sample volume increase peak area increase and width of peak increase and finally it reduce purity of our sample . So how we can judge how much sample we have to take for good result?

Answer / surya prakash

IN GC split ratio is kept high ... 1;25----1;50 ........
for good results'
'
'
'
but it all depends upon the procedure for its done ... i.e.,

1. development process or
2. for already developed method's

what is difference between Performance check and Calibration?

1 Answers  

Why we don't add Hcl, HNO3, H3PO4 in residue on ignition, why we add H2SO4

1 Answers  

what is impurity profile. how to interpret this impurity profile to a drug product or drug substance.

0 Answers  

What the difference in loss on drying and residue on ignition

2 Answers  

why we are using hexane in calibration of number of drop per mL

0 Answers  

In Stability testing if significant change occurs then what will be the action plan?

2 Answers   Cipla,

principle of AAS & its uses ?

1 Answers   Auriga,

Why potassium dichromate is used in calibration of uv?

0 Answers  

how to calculate corelation coefficient in G.C Calibration? what is the calculation part?

1 Answers   Cipla, Ortin Labs,

what is third integration

0 Answers   Orchid,

How to establish relative response factor for hplc. Why it is required?

0 Answers  

why hexadecane is used in calibration of FID IN GC THERE IS NO REMAINING SOLVENTS

1 Answers   DRL, Hetero, MSN,