Answer / k.raveendra reddy

weigh accurately about 0.8 g of anhydrous Sodium Carbonate, previously heated at about 270°C for 1 hour. Dissolve it in 100 ml of water and add 0.1 ml of methyl red solution. Add the acid slowly from a burette, with constant stirring, until the solution becomes faintly pink.

How do we fix the sample concentaryion in hplc method development. what is the basis?Thanks in advance

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