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In UV we measure absorbance , how it is happend ? explain me
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Answer / rajeev ranjan
IN UV there is "transition of electron".During transition Of electron from lower orbit to higher orbit it absorbs uv radiation following basic principle Beers Lambert law
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Answer / durgaprasad
electron transision from ground stage to excited stage
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Answer / vilas
Uv radiation absorb by electron of specific sample.for excited stage they release energy . It causes absorption & trasmitance.
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what is the difference between the Purity and Potency of drug substance
1 Answers Divis Laboratories, Graviti Pharmaceuticals,
what is the value of pH
why does used dry caffien in HPLC calibration?
How long we can use hygroscopic reference standard in terms of water absorb.I mean in line with respective monograph's specification or more than that?
what is principal of karl ficher instrument!
who is the father modern liqiud chromatogrphy?
3 Answers Amneal Pharmaceuticals, Car Chem, Cipla,
In HPLC calibration, caffeine is used as primary standard for wave length calibration due to caffeine is having dual maxima at 273 & 205 nm and one minima at 245 nm. Any body can give reference of these details from any pharmacopeia (with chapter no.) or any other authentic guideline?
which are the sizes of capsules?
What is Spliit and Splitless in Gas Chromatography nad How does it work?
How to establish pKa for a new API molecule ?
2 Answers Neuland, Sun Pharma,
For standardization of volumetric solutions, acceptance limit for concentration is considered as 10% of nominal concentration and %RSD as NMT 0.2% (for appropriate no. of replicates). But , if %RSD has not met, what should be further action?
why we use caffeine for hplc calibration?
Organic Chemistry 
