Explain in detail what are the procedure and calculations
involved for accuracy (%Recovery)in method validation?
Amount recovered (µg)
% Recovery = -------------------------X 100
Actual amount added (µg)
AreaSampleX Wt.Std X Potency
Amount recovered(µg)= ----------------------------
Area of Standard x 100
Amount added (µg) X Potency
Actual amount added(µg)= ----------------------------
100
Weight of std taken for recovery stock(mg)
( X Recovery Stock Solution addaed (ml)X1000
Amount added(µg)= ---------------------------------------
10
Is This Answer Correct ? | 1 Yes | 4 No |
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4 Answers BioCan, Biocon, Megafine Pharma,
hii, i'm had a sample of 1L that gone through extraction & evaporation. i used 0.5ml of methanol for reconstitution before injecting the sample to HPLC. i made a calibration curve using a standard solution but now i'm confused about how to display the concentration i got from the sample. for ex. i got 0.2ug/ml how i'm gonna explane this is for the 1L ? please help .. i'll really appreciate it thanks
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