what is a differance between assay by HPLC and assay by
titration?
Answer Posted / supriya
1. Some of the compounds only detected in manual titrations based on colour changed.
2.some of the compounds are detected by electrode by autotitrator.
3.Some of the compounds(we should not able to identifie the end point by adding indicator) will respond only Based on the nanometer we can analyse by Assay by HPLC these type of compounds are not responded by the indicators.
The electrode is also nothing but one type of indicator.The electrode will respond Based on the milli volts and pH,there is no need to color identification(mens end point).
Is This Answer Correct ? | 2 Yes | 2 No |
Post New Answer View All Answers
Hi sir if any product monograph not given known impurities (may be 5 impurities) specifications then how we require to proceed for inhouse formulation?
How to decide assay range for non pharmacopeial API analysis by HPLC? Could you give me any reference for same e.g Guidelines or Paper publications?
In IR spectra, generally a molecule vibrates without apply emr or ir light?
please explian me about area normalisation method,diluted standard method,impurity vs impurity and which one has to be selected in method development
We use Potassium Dichromate solution for the calibration of UV-Visible Spectrophotometer in UV region. My question is to calibrate visible region which solution could be used in photometric accuracy
why multimedia dissolution require to do?
How can I develope method of dissolution by HPLC OR UV
2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.
what is difference between UV - VISIBLE MODEL NO like 1600,1601,1700 etc ? plz explain me
How can we calculate offset value pH measurment
why glutent are detected in the rice cereal baby food product even manufacturer claimed that they are using rice and milk only?we have using ELISA to do the test,and rice supposed not containing any glutent,rite?We already repeat the test so many times and it still detected.just wondering where the glutent came from?
about method developement in hplc
why cone formation during dissolution?
give clarity of linearity and range in method validation
WHY pKa value is more important than pH value of the mobile phase in HPLC?