what is a differance between assay by HPLC and assay by
titration?

Answer Posted / supriya

1. Some of the compounds only detected in manual titrations based on colour changed.
2.some of the compounds are detected by electrode by autotitrator.
3.Some of the compounds(we should not able to identifie the end point by adding indicator) will respond only Based on the nanometer we can analyse by Assay by HPLC these type of compounds are not responded by the indicators.
The electrode is also nothing but one type of indicator.The electrode will respond Based on the milli volts and pH,there is no need to color identification(mens end point).

Is This Answer Correct ?    2 Yes 2 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

Hi sir if any product monograph not given known impurities (may be 5 impurities) specifications then how we require to proceed for inhouse formulation?

278


How to decide assay range for non pharmacopeial API analysis by HPLC? Could you give me any reference for same e.g Guidelines or Paper publications?

2863


In IR spectra, generally a molecule vibrates without apply emr or ir light?

1433


please explian me about area normalisation method,diluted standard method,impurity vs impurity and which one has to be selected in method development

1098


We use Potassium Dichromate solution for the calibration of UV-Visible Spectrophotometer in UV region. My question is to calibrate visible region which solution could be used in photometric accuracy

1315






why multimedia dissolution require to do?

308


How can I develope method of dissolution by HPLC OR UV

1893


2. Two grams of Benzoic acid are dissolved in 200 ml of water and extracted with 200 ml of diethyl ether. The distribution coefficient of benzoic acid is 100, and its dissociation constant is 6.5 10-5. Calculate the distribution ratio (D) of benzoic acid at pH 2, 5, and 6. 3. Calculate D at pH 2 to 10 (1 unit apart) in the above problem, and plot D versus pH.

1760


what is difference between UV - VISIBLE MODEL NO like 1600,1601,1700 etc ? plz explain me

2221


How can we calculate offset value pH measurment

1069


why glutent are detected in the rice cereal baby food product even manufacturer claimed that they are using rice and milk only?we have using ELISA to do the test,and rice supposed not containing any glutent,rite?We already repeat the test so many times and it still detected.just wondering where the glutent came from?

2341


about method developement in hplc

1213


why cone formation during dissolution?

323


give clarity of linearity and range in method validation

1860


WHY pKa value is more important than pH value of the mobile phase in HPLC?

1254