what is the peak purity concept?

Answers were Sorted based on User's Feedback

what is the peak purity concept?..

Answer / raghavaiah mandalapu

Peak purity analysis is an evaluation technique for
detecting the presence of coeluting impurities in HPLC
data. Running a peak purity check prior to analytical
quantitation helps to ensure accuracy. In the development
of analytical methods, peak purity analysis can reveal the
presence of contamination during standardization and, by
doing so, can prevent the subsequent generation of false
analytical data.

Peak purity analysis is also a useful addition to routine
quality control procedures, especially in the analysis of
pharmaceuticals and food products, for which contamination
and quality of results are critical.

Is This Answer Correct ?    21 Yes 2 No

what is the peak purity concept?..

Answer / rahul jain

The concept of Peak purity is generally used in HPLC using PDA detector. PDA detector scan a single peak at various points, if peak is pure than the uv spectrum of peak at different points will be same but if peak is not pure than uv spectrum of peak at different points will not be same.

Is This Answer Correct ?    13 Yes 3 No

what is the peak purity concept?..

Answer / king_suresh-chelika

Peak purity concept is spectrally homogeneity of a perticular peak, if two or more peaks having same polarity, then they will come at same RT (most probably some times merge with each other)
Even though they have different uv absorption, due to same polarity, peaks merged or co elution observed.
In this case, angle will be higher than threshold,results peak purity fails

Is This Answer Correct ?    1 Yes 0 No

what is the peak purity concept?..

Answer / raghavaiah mandalapu

A technique is described whereby the purity of an HPLC peak
can be determined by using electrospray liquid
chromatography-electrospray ionization mass spectrometry.
Electrospray mass spectra acquired across an HPLC peak are
summed and examined for co-eluting impurities. The mass
spectrometer is set up to produce solely cationised
molecules and background noise is minimized so that minor
coeluting impurities can be observed down to a level of
<0.1% of the major component. This method offers advantages
over using diode-array UV detection (LC-DAD) for the
determination of HPLC peak purity, namely components with
similar UV spectra can be distinguished, the molecular mass
of the impurity can be determined and structural data can
be obtained by using tandem mass spectrometry (MS-MS). The
effectiveness of the technique is demonstrated with drug
substances of pharmaceutical interest which have been
chromatographed on an HPLC system designed to intentionally
co-elute a number of impurity standards with these

Is This Answer Correct ?    4 Yes 4 No

what is the peak purity concept?..

Answer / v.rambabu

The particular peak does not coeluted with other impurities

purity= The ratio of absarbance between the root of up
slope and apex/The ratio of absarbance between the root of
down slope and apex

Is This Answer Correct ?    2 Yes 3 No

Post New Answer

More Analytical Chemistry Interview Questions

How to Perform a Recrystallization ?

4 Answers  


0 Answers   Reddy Labs,

in pentoperazole capsules usp why assay value is substract from dissolution

1 Answers   Ranbaxy,

what is differance between volatile and non volatile compound expalin by exaple

2 Answers  

Why do we use KMnO4 in the test of control of obsorbance ? and why do we take specific quantity i.e 57-63mg?

0 Answers   Reddy Labs, Vimta Labs,

one gram of which below elements has the most atoms in its? a) H b) D C)T D) He

4 Answers  

What is quality controle.

6 Answers  

why we use deturium lamp in uv spctrophotometer other than lamps

1 Answers  

What is pooled sample in Dissolution

2 Answers   Lupin, Graviti Pharmaceuticals,

When we calculate Impurity against impurity std.solution and when we calculate impurity against diluted standard with applying RRF?

2 Answers   Torrent Pharma,

what is poarimeter, why it is used in pharma,and explain briefly about polarimeter?

3 Answers   GVK,

How to fix the concentration for RS by HPLC

8 Answers   Reddy Labs, Intas,

  • Organic Chemistry Interview Questions Organic Chemistry (289)
  • Inorganic Chemistry Interview Questions Inorganic Chemistry (113)
  • Analytical Chemistry Interview Questions Analytical Chemistry (1223)
  • Physical Chemistry Interview Questions Physical Chemistry (62)
  • General Chemistry Interview Questions General Chemistry (300)
  • Chemistry AllOther Interview Questions Chemistry AllOther (77)