Interested to Buy Any Domain ? << Click Here >> for more details...
same sample on two preapration gives differnt result during
hplc analysis
- 3 Answers
- 8279 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / hanimi
Before conclusion we have to review the data. investigation
has to done on test method and on experiment.
if there is no problem in experiment. It may have two
reasons
1. Analytical method is not suitable to get the the
consistant results
2. Sample is not uniform
| Is This Answer Correct ? | 10 Yes | 0 No |
Answer / reet
there may be any one of the reasons:
1. Air bubble in the injector needle (needle wash 2-3 times0
2. carry over might be there.
3. random variation in the flow rate
4. Integration problem (chk the processing method)
5. Sample itself may a problem !
i. solubility issues
ii.sample(generally proteins may be sticking to the
walls of the sample vial and also may be getting
irreversibly stuck on the surface of the column)
iii. temperature change.
| Is This Answer Correct ? | 5 Yes | 0 No |
what v define from IR & NMR spectroscopy ?
in which situation require to change rs specification?
what is range of Vivible UV ? What is UV& Vivible spctroscopy use for ?
HPLC Calibration was done in Reverse Phase, but not in normal phase why explain?
how to regulate noise in hplc ?
What is difference between known and unknown impurities
How much is the standard area for glc analysis
state the principle of gas chromatography?
How to select Sample concentration in RS analysis?example.
what are the standard methods for HPLC?
in karl fischer reagent which solvent is used for dissolving iodine sulphur dioxide and imidazole
1 why polyesterene film used for ftir calibration ? 2 why k2cr2o7 is used for control of abs in uv ? 3 why holmium oxide used for control of wavelength in uv
Organic Chemistry 
