same sample on two preapration gives differnt result during
hplc analysis
Answer Posted / reet
there may be any one of the reasons:
1. Air bubble in the injector needle (needle wash 2-3 times0
2. carry over might be there.
3. random variation in the flow rate
4. Integration problem (chk the processing method)
5. Sample itself may a problem !
i. solubility issues
ii.sample(generally proteins may be sticking to the
walls of the sample vial and also may be getting
irreversibly stuck on the surface of the column)
iii. temperature change.
Is This Answer Correct ? | 5 Yes | 0 No |
Post New Answer View All Answers
If we do accuracy at same concentration at which linearity planned,what is the need to do linearity separately?
which are the sizes of capsules?
Why a1% value is used for some product ? What is the criteria for selection a1% value ?
What is the diffrence in japan mkt requirement in analytical method validation over US?
Which type of column should i use to check the purity of high molecular weight protein using HPLC reverse phase column chromatography? Hi everyone. I wanna to check the purity of high molecular weight protein (collagen) with MW of ~130 kDa using a HPLC. I know C18
For titration in anhydrous media with perchloric acide, if lack of titrator, Which indicator is been used for replacement. How calculate pH of test solution to choose suitable indicator?
if you have given a blank water how you analyse by general analytical techniques?
if peak get problamatic then what require to do?
In which situation we require to analytical method validation of excipient?
how to calculate elemental impurities?
calibrtion procedue of LC-MS
What should be the level/ Percentage of Arsenic in Arsenic free zinc.
how decide the clining method and cleaning method validation require for this perticular products?
If inhouse hplc related substance method is completly diffrent from Usp for finished proďuct with diffrent impurities then how require to prove method equivalecy?
What is the difference between chromatographic purity and related substances?