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same sample on two preapration gives differnt result during
hplc analysis
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Answer Posted / reet
there may be any one of the reasons:
1. Air bubble in the injector needle (needle wash 2-3 times0
2. carry over might be there.
3. random variation in the flow rate
4. Integration problem (chk the processing method)
5. Sample itself may a problem !
i. solubility issues
ii.sample(generally proteins may be sticking to the
walls of the sample vial and also may be getting
irreversibly stuck on the surface of the column)
iii. temperature change.
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