How did you conclude that when stressing a compond, all
degradation products have eluted in chromatogram?
Answers were Sorted based on User's Feedback
Answer / s.elumalai
The main aim of degradation study is to separate know
impurities in the case when we do not have know impurities
or to generate impurities from the sample by degradation.
Obviously one should know the process related impurities or
the degradation pathways. From peak purity by PDA or Mass
analysis one can conclude the purity of each peak eluting,
beyond that mass balance also account for peaks.If mass is
not balanced then one can conclude that some of the compound
are not eluted or may not have UV absorption.
Is This Answer Correct ? | 7 Yes | 0 No |
Answer / hemant
Method should be stability indicating & should give mass
balance
Is This Answer Correct ? | 3 Yes | 0 No |
Answer / naga
in specificity,by mass balance study we can confirm all
impurities are eluted in specified run time.
Mass balance = Assay of main peak+total net degradation
from this we can conclude what r the degradants r there all
r eluted the desired runtime.
Mass balance limit NLT 95%
Is This Answer Correct ? | 3 Yes | 0 No |
Answer / m.sivalingeswararao
By calculating mass balance.It should be equal to or nearer to 100%.%Decrease in mass balance indicates the % of Impurities not detected or not eluted. In some cases Degraded Impurities may not be eluted with in specified run time,in such cases they may elute in the next run ,hence it should be advisable to increase the run time.
Is This Answer Correct ? | 1 Yes | 0 No |
Answer / pramod bhosale
In a dregradation process there should no effect of main
drug when degradant peak elutes in degradation.
Is This Answer Correct ? | 0 Yes | 0 No |
Answer / babu.s
when we stressing a compound,all degradation products are may eluted or may not be eluted in chromatogrm.Due to stressing the form of drug my be changed to some extent. in that cases, the changed form is identified by pXRD/dsc.so better go TO UV/IR FOR BETTER RESULT.
Is This Answer Correct ? | 0 Yes | 0 No |
Answer / divya
degrading produts are considered as impurities and small in
size so they elute fast.
Is This Answer Correct ? | 1 Yes | 13 No |
why are measure gas flow " ml " in Gas chromatography
In Karl Fischer titration,What is the situation if the material tested is only soluble in water,and not soluble in the common solvents used such as methanol ?? [e.g. Iron III Hydroxide polymaltose]
Why KBR is used in IR for preparation of pellete, why it shows zero dipole moment
why using Potentometry What Purpose? differance between manual and potentiometry? why using Some of materials in usig potentiometry? What is use of mericuric acetate in Potentiometry
How to calculate the maximum loaded sample concentration in HPLC column?
how to convert normal phase hplc to reverse phase and vice versa?
how can I make the copper get white bye any salt or acit?
1 why polyesterene film used for ftir calibration ? 2 why k2cr2o7 is used for control of abs in uv ? 3 why holmium oxide used for control of wavelength in uv
what are peak purity,peak purity index,peak thresold
What is Isotope tracer technique?
1 Answers LAP, MAHINDRA, MPSC, PSI Data Systems, WNS,
WHEN AN UN KNOWN SAMPLE IS GIVEN TO U,HOW WILL U START THE ANALYSIS(ie FIRST WHICH TEST TO BE DONE,NEXT,THEN NEXT),HOW WILL U DECIDE WHICH TYPE OF ANALYSIS TO BE DONE FOR IT?
In Analytical method development related substance method when did follow w/w method when did follow area normalisation method
2 Answers Dr Reddys, Sun Pharma,