how PH an important role in HPLC separation
- 3 Answers
- 15608 Views
- I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / venkat.1830@gmail.com
acidic compounds are more retained at low ph ,basic
compounds are more retained at higher ph while neutral
compounds are uneffected. PH can be used as a powerful
selectivity tool for the separation of ionizable
compounds.At PH values used traditionally (PH 4-8 or
depending upon column specifications ),a change in PH can
result in a dramatic shift in retention
| Is This Answer Correct ? | 50 Yes | 2 No |
Answer / r.kadambavadani
The column stationary phase is also affected by pH. At very low pH (<2) the bonded stationary phase will be stripped from the silica support. At high pH (>8) the silica itself will be damaged by dissolution. At mid pH ranges (>5), any lone silanol groups (Si-O-H) present will act like an acidic moiety and will become ionized - increasing the tailing observed with analytes containing basic functional groups.
| Is This Answer Correct ? | 1 Yes | 0 No |
Answer / g.ganesh
compound is completly retaned at spacefic pH valu in the
coloum and give peak which is sharp and some time pH affect
on some compound so thair is chance to decompose or distrub
to compound stbility
| Is This Answer Correct ? | 3 Yes | 7 No |
What is impurities in pharma analysis?
Why used 0.02% v/v solution of toluene in hexane for calibration of UV.
what is the meaning of stress study?
some product having water content method with pyridine and ethylene glycol mixture instead of methanol? how they are selecting and methanol is not suitable solvent how ?
Why PH range from 0to14.
2 Answers College School Exams Tests, Reddy Labs, Wings Customer Care,
What is Standard deviation
what is stability study ? why it is needed and how itis done
Why dissolution test apparatus calibration with salycilic acid tablets was stopped by FDA&USP from the year of 2009?
what is the source of ir spectroscopy
2 Answers CTX Life Sciences, Mylan,
What is the Deference between Bulk density & Tap density ?
2 Answers Hikma Pharmaceuticals, Neuland, Reddy Labs,
For Recent draft guidelines for Dissolution test apparatus calibration, why removing the salicylate tables?
In GC Why does retention time decrease when temperature increases? Also how does a change in temperature effect selectivity?
Organic Chemistry 
