Question { 15561 }
how PH an important role in HPLC separation
Answer
acidic compounds are more retained at low ph ,basic
compounds are more retained at higher ph while neutral
compounds are uneffected. PH can be used as a powerful
selectivity tool for the separation of ionizable
compounds.At PH values used traditionally (PH 4-8 or
depending upon column specifications ),a change in PH can
result in a dramatic shift in retention