In GC and HPLC ,how we can decide which of following method will be suitable or use for quantitation of chromatogram at the time of method development. 1.Area Normalisation 2.External standard method 3.Internal standard method
Atul,
1 5605In capadity factor formula is K'= (RT of main compound/RT of first eluted peak)-1 in this formula what is 1
1 5415hii, i'm had a sample of 1L that gone through extraction & evaporation. i used 0.5ml of methanol for reconstitution before injecting the sample to HPLC. i made a calibration curve using a standard solution but now i'm confused about how to display the concentration i got from the sample. for ex. i got 0.2ug/ml how i'm gonna explane this is for the 1L ? please help .. i'll really appreciate it thanks
1 2799Dear Sir, This is Thirunavukkarasu am working in Ordain Health Care AR&D, Am Having one problem While doing method development my question Metadoxine and Pyridoxine same chemical nature and structural activity i tried lot of methods but Analyte eluted same Retantion time i cant separate both peaks. solubility, pH both are same only only difference melting point metadoxine 210 C Pyridoxine 102 C please do the help needful for me
5 6661some product having water content method with pyridine and ethylene glycol mixture instead of methanol? how they are selecting and methanol is not suitable solvent how ?
1537Post New Analytical Chemistry Questions
why we are using hexane in calibration of number of drop per mL
What is intact assay method development.....?
How can we confirm the HPLC column is end-capped or not? Is it possible to identify by physical appearance?
in gas chromatography what is the difference between gas flow rate and average linear velocity ?
My question about gas chromatography sulfur chemiluminsecence detector. I test unknown sample gas by GC-SCD (calibrated ) and the result of *H2S is 279 PPM , *but when I test the same sample with the GC-TCD (calibrated ) the value of *H2S is 2500 PPM . I'd like to inform you that both GCs are calibrated and have very good operation conditions with stable parameters . the question is if the sample gas with higher H2S over detection limits of SCD detector (1000 ppm). why the result it 279 ppm Best regards
what is partion and column chromatography
how will you do the prep for unstable componds?
how you determine moist.of NaOH sol or NaOH flacks
How would you decide dissolution medium for NCE compound of class I drug
what is the origin to prepare standard operating procedure
Why do we use KMnO4 in the test of control of obsorbance ? and why do we take specific quantity i.e 57-63mg?
Which type of column should i use to check the purity of high molecular weight protein using HPLC reverse phase column chromatography? Hi everyone. I wanna to check the purity of high molecular weight protein (collagen) with MW of ~130 kDa using a HPLC. I know C18
In HPLC (Waters) software Empower1,2 &3 what is the updation for each version
While performing TOC sst analysis Zero shift disabled & sample analysis zero shift enabled why?
how can give the expiry period and restadardisation of volumetric solution
