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In GC and HPLC ,how we can decide which of following method will be suitable or use for quantitation of chromatogram at the time of method development. 1.Area Normalisation 2.External standard method 3.Internal standard method
Atul,
1 7281In capadity factor formula is K'= (RT of main compound/RT of first eluted peak)-1 in this formula what is 1
1 7075hii, i'm had a sample of 1L that gone through extraction & evaporation. i used 0.5ml of methanol for reconstitution before injecting the sample to HPLC. i made a calibration curve using a standard solution but now i'm confused about how to display the concentration i got from the sample. for ex. i got 0.2ug/ml how i'm gonna explane this is for the 1L ? please help .. i'll really appreciate it thanks
1 4219Dear Sir, This is Thirunavukkarasu am working in Ordain Health Care AR&D, Am Having one problem While doing method development my question Metadoxine and Pyridoxine same chemical nature and structural activity i tried lot of methods but Analyte eluted same Retantion time i cant separate both peaks. solubility, pH both are same only only difference melting point metadoxine 210 C Pyridoxine 102 C please do the help needful for me
5 9731some product having water content method with pyridine and ethylene glycol mixture instead of methanol? how they are selecting and methanol is not suitable solvent how ?
2655Post New Analytical Chemistry Questions
HI,I CLEARED BOB CLERK EXAM. MY INTERVIEW WILL BE ON 9TH OCTOBER,2010.PLEASE SEND ME INTERVIEW QUESTIONS AND ANSWERS. THANK YOU.
what is mean by covalidation
Hi sir if any product monograph not given known impurities (may be 5 impurities) specifications then how we require to proceed for inhouse formulation?
Please give idea about method development for cleaning method and how maco level establish
I have compare C2H2-air and C2H2-N2O flame AAS on determination calcium. Both use same range of std to plot calibration curve. (2-6ppm) When i measure the sample with phosphate, KCl and LaCl, C2H2-N2O flame give false positive result, around 0.5ppm. When i measure the sample with phosphste, KCl and EDTA. C2H2-N2O flame also give 0.5ppm false positive. But both above mentioned sample would not give false positive when measured by C2H2-air flame. What is the reason?
What is similarity factor and its use in api strength?
what are the standard methods for HPLC?
which situation gc hs and gc als require to use?
While performing TOC sst analysis Zero shift disabled & sample analysis zero shift enabled why?
what is difference in ods I ods II is this is realy from sorbent / packing characteristics or from other means
How to choose the which salt is suitable for mobilephase
Why we select scan range from higer wavelength to lower wavelength in uv visible spectroscopy and ftir spectroscopy ? .
in api coa contains only process impurities and in product coa contains degradation impurities?
why should we perform dissolution PVT calibration only by UV spectrometer, not by HPLC ?
Why only Copper standard is used to calibrate Atomic Absorption spectrophotometer?
