In GC and HPLC ,how we can decide which of following method will be suitable or use for quantitation of chromatogram at the time of method development. 1.Area Normalisation 2.External standard method 3.Internal standard method
Atul,
1 6525In capadity factor formula is K'= (RT of main compound/RT of first eluted peak)-1 in this formula what is 1
1 6244hii, i'm had a sample of 1L that gone through extraction & evaporation. i used 0.5ml of methanol for reconstitution before injecting the sample to HPLC. i made a calibration curve using a standard solution but now i'm confused about how to display the concentration i got from the sample. for ex. i got 0.2ug/ml how i'm gonna explane this is for the 1L ? please help .. i'll really appreciate it thanks
1 3475Dear Sir, This is Thirunavukkarasu am working in Ordain Health Care AR&D, Am Having one problem While doing method development my question Metadoxine and Pyridoxine same chemical nature and structural activity i tried lot of methods but Analyte eluted same Retantion time i cant separate both peaks. solubility, pH both are same only only difference melting point metadoxine 210 C Pyridoxine 102 C please do the help needful for me
5 8090some product having water content method with pyridine and ethylene glycol mixture instead of methanol? how they are selecting and methanol is not suitable solvent how ?
2093Post New Analytical Chemistry Questions
how we can identify the impurity is coming below loq at transfering site?
i want model written test paper for the post of analyst in IOCL(indian oil corporation ltd) any one pls help me
in which situation require to change rs specification?
Why we select scan range from higer wavelength to lower wavelength in uv visible spectroscopy and ftir spectroscopy ? .
what is mean by ambient temperature?
[3/30, 13:29] Manoj P Venkatpurwar: How hplc column selection according to structure? How mobile phase buffer selection on molecule structure?
how can I make the copper get white bye any salt or acit?
how require to select dissolution media? what is discrimination?
why multimedia dissolution require to do?
[3/28, 20:52] Manoj P Venkatpurwar: how many impurities require to inject in assay specificity that how we can find out? and in Rs also how?
in which situation ion pair agent require to use?
how require to set assay concentration for standard and sample?
what parameters we will consider while developing a HPLC method and how we confirm our HPLC method is valid?
what is the importence and role of LCMS and LCMS/MS and their applications? which type of compounds are analysis by lcms?
What is the formula for relative diffrence for standard solution in solution stability in validation?
