In GC and HPLC ,how we can decide which of following method will be suitable or use for quantitation of chromatogram at the time of method development. 1.Area Normalisation 2.External standard method 3.Internal standard method
Atul,
1 6194In capadity factor formula is K'= (RT of main compound/RT of first eluted peak)-1 in this formula what is 1
1 5870hii, i'm had a sample of 1L that gone through extraction & evaporation. i used 0.5ml of methanol for reconstitution before injecting the sample to HPLC. i made a calibration curve using a standard solution but now i'm confused about how to display the concentration i got from the sample. for ex. i got 0.2ug/ml how i'm gonna explane this is for the 1L ? please help .. i'll really appreciate it thanks
1 3156Dear Sir, This is Thirunavukkarasu am working in Ordain Health Care AR&D, Am Having one problem While doing method development my question Metadoxine and Pyridoxine same chemical nature and structural activity i tried lot of methods but Analyte eluted same Retantion time i cant separate both peaks. solubility, pH both are same only only difference melting point metadoxine 210 C Pyridoxine 102 C please do the help needful for me
5 7518some product having water content method with pyridine and ethylene glycol mixture instead of methanol? how they are selecting and methanol is not suitable solvent how ?
1827Post New Analytical Chemistry Questions
Why we used in n-butyl acetate water content terminology while in ethyl acetate we used moisture content terminology?
How can the GC or HPLC method is selected to determine the impurity profile in drug product?
how require to set assay concentration for standard and sample?
what is mean by ambient temperature?
pharma industries oriented
Hi sir if any product monograph not given known impurities (may be 5 impurities) specifications then how we require to proceed for inhouse formulation?
In BP2013, Loperamid HCl monograph. Assay by titration with 0.1N sodium hydroxide using hydrocloric acid 0.01N and reading the volume added between the 2 points of inflexion. I have a question that if the diluent solvent is ethanol is certainly consumed a amount of volume of titrant, so this volume must be eliminated on the result calculation or not apart from first point which is subtracted above.
why lactose has less charges when packed in steel containers when compared with packing of lactose in polyamide material?
In which situation we require to prepare the standard solution from sample in Related substance method?
what is the acceptance criteria for enteric coated tablets in 0.1n hcl validation in each parameter?
What is the principle of Thermal conductivity detector and FID?
how a particular wavelength can be different for a particular compund while analysing by uv and by HPLC.
What is the main difference between ODS1 and ODS2 Hplc column.
please explain about aluminium hydroxide assay
What is shaking level in GC?
