why we conclude the area% for the chromatographic purity?

Answer Posted / prakash

1.the absorbance of the analyte peak should not be saturate either below or above 1000 milli absorance Unit.
2. if go for diluted standard level the analyte peak absorbance response should same compare to your high level absorbance response.
3. if you calculate the result either %area normalization or diluted standard level the result should same.
in this case, only we can proceed %area normalization method.

Is This Answer Correct ?    2 Yes 0 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

What is the difference between chromatographic purity and related substances?

774


WHICH STANDARD WE USE IN SILICA ANALYSIS IN SILICA-GEL METHOD FOR SOLID SAMPLE

1609


What is the difference between purge septum flow and column flow in gas chromatography?

1823


WHAT IS THE CRITERIA FOR SELECTION OF TIME OF DISSOLUTION AND THE MEDIUM OF DISSOLUTION?

2132


What is the main difference between ODS1 and ODS2 Hplc column.

4459






what type of questions asked on analytical balance and also give answers................all pharma companies?

11709


Why are use silicon oil mr?

734


what are the standard methods for HPLC?

2236


Principle of single pan analytical balance

4590


what is the acceptance criteria for enteric coated tablets in 0.1n hcl validation in each parameter?

331


How to do regeneration of Metacarb Pb plus column?

2071


is it necessary to do HPLC calibration at wavelength 315nm if we are doing analysis at this wavelangth

1652


How to set analytical specification for combination products?

314


[3/28, 20:52] Manoj P Venkatpurwar: how many impurities require to inject in assay specificity that how we can find out? and in Rs also how?

337


What is delay volume?

836