Follow Our FB Page << CircleMedia.in >> for Daily Laughter. We Post Funny, Viral, Comedy Videos, Memes, Vines...


what is use of acetonitrile compare to methanol in RP-HPLC
Method devlopment

Answers were Sorted based on User's Feedback



what is use of acetonitrile compare to methanol in RP-HPLC Method devlopment..

Answer / sandesh

the uv cut oof value of methanol is 210 nm so we cant use
methanol near about lambda max of 200 nm. so prefer to ACN

Is This Answer Correct ?    28 Yes 4 No

what is use of acetonitrile compare to methanol in RP-HPLC Method devlopment..

Answer / balaji

the uv cut oof value of methanol is 210 nm so we cant use
methanol near about lambda max of 200 nm. so prefer to ACN
& ACN is highly polar as compare to Methanol So provide
Better resolution for many compound and it has property to
form hydrogen bond so provide better selectivity.

Methanol viscosity more than the ACN. so ACN easly flow
through column

Is This Answer Correct ?    15 Yes 1 No

what is use of acetonitrile compare to methanol in RP-HPLC Method devlopment..

Answer / dharmesh radadiya

ACN is highly polar as compare to Methanol So provide Better
resolution for many compound and it has property to form
hydrogen bond so provide better selectivity.

Is This Answer Correct ?    17 Yes 10 No

what is use of acetonitrile compare to methanol in RP-HPLC Method devlopment..

Answer / bharat patel

same as answer 4

Is This Answer Correct ?    6 Yes 1 No

what is use of acetonitrile compare to methanol in RP-HPLC Method devlopment..

Answer / snehal

ACN is more polar and neutral solvent and has low viscosity than methanol hense shows low back pressure than methanol

Is This Answer Correct ?    7 Yes 3 No

what is use of acetonitrile compare to methanol in RP-HPLC Method devlopment..

Answer / guest

strength of elution varies among different organic solvents...among methanol and acetonitrile, acn has higher elution strength than methanol

Is This Answer Correct ?    1 Yes 3 No

what is use of acetonitrile compare to methanol in RP-HPLC Method devlopment..

Answer / rohithirpara

Dear Dharmesh
Methanol is very polar than ACN

Is This Answer Correct ?    7 Yes 28 No

Post New Answer

More Analytical Chemistry Interview Questions

Which column is more polar, C8 or C18?

6 Answers   Cipla, Glenmark, Laurus, Torrent Power,


wat is the role of michelson interferometer in FTIR and He-Ne lase.detailed answer required in simple language

1 Answers   Police Constable,


What is primary & Secondary Standard ? Give Example

17 Answers   Cipla, Lupin,


Why only 1.2 million lux hours require in photostability study?

0 Answers  


IS THERE ANYWHERE MENTION THAT THE RE PROCESS BATCH REQUIRE TO KEPT STABILITY STYDY

2 Answers   Nakoda Chemicals,






What do you mean by potency in Analytical industry

3 Answers  


We use Potassium Dichromate solution for the calibration of UV-Visible Spectrophotometer in UV region. My question is to calibrate visible region which solution could be used in photometric accuracy

0 Answers  


why we do the calibration of limit of stry light in hplc & uv

1 Answers   Cipla,


In Stability testing if significant change occurs then what will be the action plan?

2 Answers   Cipla,


How to prepare 10% HCl from 36.5% HCl solution.

5 Answers   Dr Reddys,


What is the name of the detector used in TOC?

1 Answers   Lupin,


why we are doing seal wash and needle wash?

3 Answers  


Categories
  • Organic Chemistry Interview Questions Organic Chemistry (302)
  • Inorganic Chemistry Interview Questions Inorganic Chemistry (123)
  • Analytical Chemistry Interview Questions Analytical Chemistry (1385)
  • Physical Chemistry Interview Questions Physical Chemistry (62)
  • General Chemistry Interview Questions General Chemistry (478)
  • Chemistry AllOther Interview Questions Chemistry AllOther (190)