BIOCHEMICAL ENGINEERING INSTRUMENTATION - EXAMPLE 29.4 : The resolution of separation, Rs for chromatography is given by the formula Rs = (difference in retention time) / (average width at the base). In a chromatogram, 3 peaks a, b and c are found. Average widths W at the bases of the solutes are : Wa = 20 s, Wb = 40 s, Wc = 30 s. Resolutions of separation, Rs for solutes b and c in comparison to a are 2 and 4 respectively. The differences in retention times T for b and c in comparison to a are (Tb - Ta) and (Tc - Ta), Ta = Tc - Tb : (a) Form 2 equations involving Rs as a function of Wa, Wb, Wc, Ta, Tb and Tc. (b) Find the values of Ta, Tb and Tc.
BIOCHEMICAL ENGINEERING INSTRUMENTATION - ANSWER 29.4 : Reference formula Rs = (difference in retention time) / (average width at the base) is used. (a) First equation : Rs = (Tb - Ta) / [ (Wa + Wb) / 2 ] = 2 (Tb - Ta) / (Wa + Wb). Second equation : Rs = (Tc - Ta) / [ (Wa + Wc) / 2 ] = 2 (Tc - Ta) / (Wa + Wc). (b) Substitute Ta = Tc - Tb, Wa = 20 s, Wb = 40 s and Wc = 30 s into first equation and second equation. First equation : Rs = 2 = 2 [ Tb - (Tc - Tb) ] / (20 + 40) = (2 Tb - Tc) / 30, 2 Tb - Tc = 60. Second equation : Rs = 4 = 2 [ Tc - (Tc - Tb) ] / (20 + 30) = Tb / 25, Tb = 100 s. Substitute Tb = 100 s into first equation gives 2 Tb - Tc = 2 x 100 - Tc = 200 - Tc = 60, then Tc = 200 - 60 = 140 s. Then Ta = Tc - Tb = 140 - 100 = 40 s. The answer is given by Kang Chuen Tat; PO Box 6263, Dandenong, Victoria VIC 3175, Australia; SMS +61405421706; chuentat@hotmail.com; http://kangchuentat.wordpress.com.
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MY generator water cooler inlet line height - 11 mtr inlet water line height - 7 mtr water come into the line from overhead tank, O/H tank height - 20 mtr the first tapping in the inlet line is 2" line which down to 4 mtr level plateform., second tapping in inlet water line goes to a pump 6" line installed at 4 mtr level plateform, pump capacity 200m3/hr, head- 30 mtr, hp-40. the outlet of pump of 6" water line connected to a header of 6 mtr long of 6" line, on this header the six tapping are connected, 4 nos. 3" line goes to 11 mtr height water cooler the pressure of the line is 0.15 to 0.25 kg/cm2, 2" and 4" pipe line connected to a cooler at 4 mtrlevel plateform. my question is, at 11 mtr height level the quantity of water and flow is not sufficient for my cooler, the temp. of cooler is rises much more. you are requested to suggest the modification of pipe line/ pump for take more water flow and pressure (desired pressure is 1 kg/cm2)
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