Question 92 - (a) In order to determine the slurry volume (L) in chromatography, arrange the formula using the following parameters : slurry concentration (%), slurry volume (L), settled chromatography medium volume (L), 100. (b) In order to determine the chromatography medium volume (L) in gel filtration chromatography, arrange the formula using the following parameters : sample volume to be processed (L), chromatography medium volume (L), sample volume in percent of total column volume (%), 100.
Answer 92 - (a) By fraction of concentration, [ settled chromatography medium volume (L) ] / [ slurry volume (L) ] = slurry concentration (%) / 100. Then [ slurry volume (L) ] = [ settled chromatography medium volume (L) ] / [ slurry concentration (%) / 100 ]. (b) By fraction of volume, [ sample volume to be processed (L) ] / [ chromatography medium volume (L) ] = sample volume in percent total volume (%) / 100. Then chromatography medium volume (L) = [ sample volume to be processed (L) ] / [ sample volume in percent of total column volume (%) / 100 ]. The answer is given by Kang Chuen Tat; PO Box 6263, Dandenong, Victoria VIC 3175, Australia; SMS +61405421706; chuentat@hotmail.com; http://kangchuentat.wordpress.com.
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Question 86 - (a) Male with genotype GGmm and phenotype gray wingless mates with female with genotype ggMM and phenotype black winged in fruit flies. G is dominant to g in color. M is dominant to m in wing shape. If the actual distribution of the second generation of the fruit flies was as follow : 890 gray wingless, 900 black winged, 115 gray winged, 95 black wingless, calculate the recombination frequency betwen the two genes and distance in recombination units. Let 1 map unit = 1 % recombination. (b) A DNA molecule has 180 base pairs and 20 % adenine. How many cytosine nucleotides are present in this molecule of DNA?
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ACCOUNTING AND FINANCIAL ENGINEERING - EXAMPLE 34.13 (CORRECTION) : (i) In the Present Value Multiplication Rule, let PV = present value, Ra = interest rate for first discount, A = duration for first discount; Rc = interest rate for second discount, C = duration for second discount. Let PV = [ 1 / (1 + Ra) ^ A ] [ 1 / (1 + Rc) ^ C ] where ^ is the symbol of power : 3 ^ 2 = 3 x 3, 2 ^ 3 = 2 x 2 x 2. (a) For discounts involving 8 % / year for 3 years and 10 % / year for 9 years, find the value of PV. (b) If Re = interest rate for third discount, E = duration of third discount, form a mathematical equation of PV as a function of A, C, E, Ra, Rc, Re. Note : Discounts are available in the purchase of certain biochemical engineering instruments. (ii) Let R = nominal interest rate related to growth rate of money, r = real interest rate related to growth rate of purchase power. If I = inflation, where the unit of R, r and I is %, find the mathematical relationship of r as a function of R and I.
Question 101 - (a) Let | A > = (Aa Ab Ac), | B > = (Ba Bb Bc), | C > = (Ca Cb Cc). Find | A > + | C > - | B > in term of Aa, Ab, Ac, Ba, Bb, Bc, Ca, Cb and Cc. (b) Let d | E > = d (Ea Eb Ec) = (d Ea d Eb d Ec). If | E > = (6 7 8), find the value of 10 | E >.
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