Question 92 - (a) In order to determine the slurry volume (L) in chromatography, arrange the formula using the following parameters : slurry concentration (%), slurry volume (L), settled chromatography medium volume (L), 100. (b) In order to determine the chromatography medium volume (L) in gel filtration chromatography, arrange the formula using the following parameters : sample volume to be processed (L), chromatography medium volume (L), sample volume in percent of total column volume (%), 100.
Answer 92 - (a) By fraction of concentration, [ settled chromatography medium volume (L) ] / [ slurry volume (L) ] = slurry concentration (%) / 100. Then [ slurry volume (L) ] = [ settled chromatography medium volume (L) ] / [ slurry concentration (%) / 100 ]. (b) By fraction of volume, [ sample volume to be processed (L) ] / [ chromatography medium volume (L) ] = sample volume in percent total volume (%) / 100. Then chromatography medium volume (L) = [ sample volume to be processed (L) ] / [ sample volume in percent of total column volume (%) / 100 ]. The answer is given by Kang Chuen Tat; PO Box 6263, Dandenong, Victoria VIC 3175, Australia; SMS +61405421706; chuentat@hotmail.com; http://kangchuentat.wordpress.com.
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QUANTUM CHEMISTRY AND CHEMICAL ENGINEERING - EXAMPLE 31.2 : (a) Let | - > = 1 | x > + 0 | y >, | | > = 0 | x > + 1 | y >. Find the value of 2 | x > + 3 | y > in term of | - > and | | >. (b) Let m to be the reduced mass. Find the value of m in term of Ma and Mb where 1 / m = 1 / Ma + 1 / Mb.
QUANTUM BIOLOGY - EXAMPLE 33.8 : (a) Let ^ be the symbol of power where 1 ^ 2 = 1 x 1 = 1, 2 ^ 2 = 2 x 2 = 4. Let the number of electrons in a human body to be 10 ^ 28 = A, the number of all of the grains of sand on Earth planet to be 7 x (10 ^ 20) = B, the number of all the stars in the visible sky to be 8 x (10 ^ 3) = C. By assuming that every star in the visible sky has the same number of grains of sand as on Earth planet, prove by mathematical calculations that there are more electrons in one human body compared to the number of all of the grains of sand on the stars in the visible sky. (b) The incoming solar radiation to the Earth's surface is mainly from sun. Around 51 % of the radiation is absorbed by Earth's surface. Around 19 % is absorbed by atmosphere and clouds. In term of reflection, 4 % of the radiation is from surface of Earth, 6 % is reflected by atmosphere and the rest is reflected by clouds. Find the percentage of radiation absorbed by and reflected by biological beings on Earth, with reason for your response.
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