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Question 92 - (a) In order to determine the slurry volume (L) in chromatography, arrange the formula using the following parameters : slurry concentration (%), slurry volume (L), settled chromatography medium volume (L), 100. (b) In order to determine the chromatography medium volume (L) in gel filtration chromatography, arrange the formula using the following parameters : sample volume to be processed (L), chromatography medium volume (L), sample volume in percent of total column volume (%), 100.
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Answer Posted / kangchuentat
Answer 92 - (a) By fraction of concentration, [ settled chromatography medium volume (L) ] / [ slurry volume (L) ] = slurry concentration (%) / 100. Then [ slurry volume (L) ] = [ settled chromatography medium volume (L) ] / [ slurry concentration (%) / 100 ]. (b) By fraction of volume, [ sample volume to be processed (L) ] / [ chromatography medium volume (L) ] = sample volume in percent total volume (%) / 100. Then chromatography medium volume (L) = [ sample volume to be processed (L) ] / [ sample volume in percent of total column volume (%) / 100 ]. The answer is given by Kang Chuen Tat; PO Box 6263, Dandenong, Victoria VIC 3175, Australia; SMS +61405421706; chuentat@hotmail.com; http://kangchuentat.wordpress.com.
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