how to correct peak when peak comes broader in shape?
How to do calibration of HPLC?
- 2 Answers
- 7300 Views
- Mankind, I also Faced
- E-Mail Answers
Answer Posted / satish
if peak is broad then use dilute solution of triethylamine to sharpen the peak
| Is This Answer Correct ? | 5 Yes | 1 No |
Post New Answer View All Answers
what is the principle involved in turbidometry and nephelometry ?
what is the structure of DOWEX-50
what is impurity profile. how to interpret this impurity profile to a drug product or drug substance.
Which products will produce when acetic anhydride reacts with Ter-butanol, Sec-butanol, Iso butanol, Dimethylamine etc.
What is gelatinization?
in each media we require to use SLS? how to proceed?
Many times I don't got a caffeine peak in calibration of hplc using guard column ❓
Ph meter can show more than 14 ph reading? Why ph range in between 1 to 14 only?
what is lod and loq ?,why use k2cr2o7 , kcl h2so4 in uv calibration ?,why use benzophenone & caffene acetone in hplc calibration ?,what is leading peak in hplc ?why we do the calibration of limit of stry light in hplc & uv ?
WHAT IS THE PARAMETER FOR THE SELECTION OF MOBILE PHASE IN HPLC?
How we performed the force degradation for drug substance, is any specific guideline is available for each parameter(Acidic, basic, oxidation,heat)? what conditions you mentained for above parameters.
1.What is the difference between method validation and method verification 2.Which guidelines proposed to method transfer
1)What's the meaning of Absorption,give a example. 2)What's the meaning of Adsorption,give a example. 2)what is the difference between Absorption and Adsorption.
Ratio of Water and Acetonitrile(diluent) is not recommended to use in cleaning method validation,why?
how to qualify the impurity?
