Answers were Sorted based on User's Feedback
Answer / laxman rapolu
where as In UV-absorbance detection : absorbance of solute
less than that of mobile phase. It can be avoided by use of
mobile phase with lower UV absorbance
|Is This Answer Correct ?||17 Yes||1 No|
Answer / falguni patel
1. Difference in the composition of the sample and mob phase.
2. Often may sample contains extraneous ingredients such as excipients.
3. Sample may not exactly dissolve in mob phase.
|Is This Answer Correct ?||0 Yes||4 No|
Answer / ramakrishna,micro labs
Based on mobile phase negative peaks we observed,for ex: the mobile phase having TEA,Acetic acid or OPA (those all r used for mobile phase pH adjustment) .that type of chemicals will show negative response in HPLC
|Is This Answer Correct ?||1 Yes||6 No|
Answer / nisha
we show negative peak because of impurites
|Is This Answer Correct ?||1 Yes||11 No|
Answer / piysh
wrong dilution selection
|Is This Answer Correct ?||3 Yes||21 No|
why sucrose is used for polarimeter calibration
How to define samples weight in moisture content
What is the purpose of performing dissolution test on routine basis?
1)why use benzophenon,uracil in hplc calibration. 2)why use sucrose in polarimeter calibration. 3)what is S/N ratio, noise and drift in hplc 4)how we can find lod and loq in method validation
which detector is used in IR
how to prepare low ppm solution from high ppm solution ?
how to calibrate hplc & gc
Why we use holmium oxide,perchloric acid for uv/vis calibration
please explain about aluminium hydroxide assay
what is the relation of normality & molarity ? give me formula?
What is the difference between specified impurity and Identified impurity
(0.41)d what is d?