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Why triethylamine and o-phosphoric acid used while setting
pH of mobile phase during HPLC Analysis?

Answers were Sorted based on User's Feedback



Why triethylamine and o-phosphoric acid used while setting pH of mobile phase during HPLC Analysis?..

Answer / g.g. krishna

Triethylamine: To reduce tailing of basic analytes
Phosphoric acid: To suppress the ionization of acidic analytes

Is This Answer Correct ?    23 Yes 2 No

Why triethylamine and o-phosphoric acid used while setting pH of mobile phase during HPLC Analysis?..

Answer / m. balaram naidu

TRI ETHYLAMIINE (AND) ORTHO-PHOSPARIC ACID ARE
USED pH ADJUSTMENT PERPASE

BECOSE
1)GOOD RESOLUTION
2)PEEK SHAPE

Is This Answer Correct ?    11 Yes 3 No

Why triethylamine and o-phosphoric acid used while setting pH of mobile phase during HPLC Analysis?..

Answer / venugopal

These are weak acid and weak base compared to
HCl,sulphuric acid, NaOH.Generally the salts used in
preparing the buffers are sodium phosphate/potassium
phosphate.Hence these reagents are added.

Is This Answer Correct ?    9 Yes 1 No

Why triethylamine and o-phosphoric acid used while setting pH of mobile phase during HPLC Analysis?..

Answer / valla priyanka reddy

triethylamine is added for good shape of peak

Is This Answer Correct ?    14 Yes 7 No

Why triethylamine and o-phosphoric acid used while setting pH of mobile phase during HPLC Analysis?..

Answer / murali krishna

Mainly Triethyl amine is used becauseof the peak Tailing and shape of the peak

Is This Answer Correct ?    4 Yes 1 No

Why triethylamine and o-phosphoric acid used while setting pH of mobile phase during HPLC Analysis?..

Answer / obaiah kade

mosty used for ph arjest

Is This Answer Correct ?    0 Yes 5 No

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