Why triethylamine and o-phosphoric acid used while setting
pH of mobile phase during HPLC Analysis?
- 6 Answers
- 34249 Views
- Pharmaceutical, Hapten Healthcare, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
Answer / g.g. krishna
Triethylamine: To reduce tailing of basic analytes
Phosphoric acid: To suppress the ionization of acidic analytes
| Is This Answer Correct ? | 23 Yes | 2 No |
TRI ETHYLAMIINE (AND) ORTHO-PHOSPARIC ACID ARE
USED pH ADJUSTMENT PERPASE
BECOSE
1)GOOD RESOLUTION
2)PEEK SHAPE
| Is This Answer Correct ? | 12 Yes | 3 No |
Answer / venugopal
These are weak acid and weak base compared to
HCl,sulphuric acid, NaOH.Generally the salts used in
preparing the buffers are sodium phosphate/potassium
phosphate.Hence these reagents are added.
| Is This Answer Correct ? | 9 Yes | 1 No |
Answer / valla priyanka reddy
triethylamine is added for good shape of peak
| Is This Answer Correct ? | 14 Yes | 7 No |
Answer / murali krishna
Mainly Triethyl amine is used becauseof the peak Tailing and shape of the peak
| Is This Answer Correct ? | 4 Yes | 1 No |
what is the difference between isocratic & elution technique?
What are the tests used in Hplc?Name any two.
why we are using benzene, anyline in acetic anhdride assay titration method?
what is the principle of assay of sodium nitrite?
Why to use propyl paraben in HPLC gradient calibration
function of detecter in hplc ,gc and spectroscopy? function of carrier gas in gc?
i want model written test paper for the post of analyst in IOCL(indian oil corporation ltd) any one pls help me
full name of OOS & OOT ? Dercribe it with details ? why this is needed in pharma industry ?
key components in gas chromotagraphy.
what is the general mechanism of high performance liqiud chromatography?
7 Answers Ajanta Pharma, Amneal Pharmaceuticals,
why potassium dicromate use in calibration of UV VIIBLE SPECTROCOPY ?
in calibration of disintegration test apparatus why cycle limit is 28-32?
Organic Chemistry 
