Why triethylamine and o-phosphoric acid used while setting
pH of mobile phase during HPLC Analysis?
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Answer / g.g. krishna
Triethylamine: To reduce tailing of basic analytes
Phosphoric acid: To suppress the ionization of acidic analytes
| Is This Answer Correct ? | 23 Yes | 2 No |
TRI ETHYLAMIINE (AND) ORTHO-PHOSPARIC ACID ARE
USED pH ADJUSTMENT PERPASE
BECOSE
1)GOOD RESOLUTION
2)PEEK SHAPE
| Is This Answer Correct ? | 11 Yes | 3 No |
Answer / venugopal
These are weak acid and weak base compared to
HCl,sulphuric acid, NaOH.Generally the salts used in
preparing the buffers are sodium phosphate/potassium
phosphate.Hence these reagents are added.
| Is This Answer Correct ? | 9 Yes | 1 No |
Answer / valla priyanka reddy
triethylamine is added for good shape of peak
| Is This Answer Correct ? | 14 Yes | 7 No |
Answer / murali krishna
Mainly Triethyl amine is used becauseof the peak Tailing and shape of the peak
| Is This Answer Correct ? | 4 Yes | 1 No |
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