how can we determine RRF value in HPLC FOR CALCULATION OF
IMPURITY ?
- 4 Answers
- 48524 Views
- Macleods, I also Faced
- E-Mail Answers
Answers were Sorted based on User's Feedback
RRF-Relative responce factor with respect to analyte(standard)
why its required- due to impurity cost,availability and time
consumption of analysis etc we can not go for everytime
impurity by impurity calculation every time
so,that finding a factor for impurities with respect to
standard.by preparing few levels of(3+ levels)standard and
impurities concentrations(can prepare between +/- 50% of
specification level of known impurities and for standard
between +/- 50% of specification level of unknown
impurities).inject the above prepared solution in to
hplc,calculate slope for impurities and standard by using
respective concentrations and areas.
RRF=SLOPE OF IMPURITIES/SLOPE OF STANDARD
| Is This Answer Correct ? | 55 Yes | 7 No |
Answer / v n murthy
RRF = IMP AREA/STD AREA*STD CONC/IMP CONC*STD PURITY/IMP
PURITY
STD AREA =MAIN PEAK AREA
| Is This Answer Correct ? | 55 Yes | 8 No |
Answer / godhani pragnesh
RRF= Relative response factor (Impurity response with
respact to main peak)
RF= Response factor (the response (e.g. peak area) of drug
substance or related substances per unit weight.
RF= peak area / concentration (mg/ml)
Relative response factor (RRF)calculation:
RRF=RF impurity / RF API
OR
RRF=slope impurity / slope API
| Is This Answer Correct ? | 42 Yes | 6 No |
Answer / vinod
In agilent hplc(ezchrome software ) we go to the report option and then right click ,the option of rrf apper add it ,it automatically apper in ur report
| Is This Answer Correct ? | 8 Yes | 38 No |
why we are using sodium,potassium phosphates for mobile phase preparation?
viod volume and dead time
How is the Flame test performed?
What is delay volume?
How do we quantify crystaline and amarpous forms by using (NMR, XRD)spectroscopic techniques? Which any others instruments are useful for this quantification? explain
0 Answers Graviti Pharmaceuticals, Vimta Labs,
why we make titiration of Iron with EDTA ?
Why red HgI2 added during starch preparation?
what is custom fields in waters system?
1 Answers Akorn India, Aurobindo,
What is the difference between chiral cel OD-H & OD-RH ?
How can we confirm the HPLC column is end-capped or not? Is it possible to identify by physical appearance?
From where require to take the RLD sample?
what is the defination of calibration & verification?
Organic Chemistry 
