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how can we determine RRF value in HPLC FOR CALCULATION OF
IMPURITY ?
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RRF-Relative responce factor with respect to analyte(standard)
why its required- due to impurity cost,availability and time
consumption of analysis etc we can not go for everytime
impurity by impurity calculation every time
so,that finding a factor for impurities with respect to
standard.by preparing few levels of(3+ levels)standard and
impurities concentrations(can prepare between +/- 50% of
specification level of known impurities and for standard
between +/- 50% of specification level of unknown
impurities).inject the above prepared solution in to
hplc,calculate slope for impurities and standard by using
respective concentrations and areas.
RRF=SLOPE OF IMPURITIES/SLOPE OF STANDARD
| Is This Answer Correct ? | 55 Yes | 7 No |
Answer / v n murthy
RRF = IMP AREA/STD AREA*STD CONC/IMP CONC*STD PURITY/IMP
PURITY
STD AREA =MAIN PEAK AREA
| Is This Answer Correct ? | 55 Yes | 8 No |
Answer / godhani pragnesh
RRF= Relative response factor (Impurity response with
respact to main peak)
RF= Response factor (the response (e.g. peak area) of drug
substance or related substances per unit weight.
RF= peak area / concentration (mg/ml)
Relative response factor (RRF)calculation:
RRF=RF impurity / RF API
OR
RRF=slope impurity / slope API
| Is This Answer Correct ? | 42 Yes | 6 No |
Answer / vinod
In agilent hplc(ezchrome software ) we go to the report option and then right click ,the option of rrf apper add it ,it automatically apper in ur report
| Is This Answer Correct ? | 8 Yes | 38 No |
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