In the method validation proceedure,lod & loq calculation we use the formulae for LOD=3.3*SD/slope and for LOQ=10*SD/slope,why we use 3.3 & 10 in the formulae?
LOD :- LOD is measured 10 times independent sample of Blank
and Student-t test of 10 times, Degree of freedom 9 (n-1)at
99% confidence label is 3.25 as per Student chart. Also
Ratio of S/N =3:1
LOQ:- LOD is regarded as the lower limit for precise
quantitative Measurement . LOQ defined as concentration
which would a produce a signal 10-12 time. At this point
standard deviation is small enough (Approximately 10-15%) so
that quantified value can be deemed reliable. Also Ratio
S/N= 10:1
Is This Answer Correct ? | 7 Yes | 3 No |
is it nessesary to do solution stability for 7 days?
what is protocol ? plz Explain me
What are the primary considerations in extractables and Leachables ?
what is classification of elemental impurities? what is risk assement in elemental impurities?
What is Signal to noise ratio?
I have got selected for Aurobindo Pharma AR&D, HR person is asking me to sign in agreement. can I join?
How do we fix the sample concentaryion in hplc method development. what is the basis?Thanks in advance
4 Answers DRL, Glenmark, Orchid,
What is use of LOD and LOQ in validtion and give range and in calculation why we use 3.3* LOD here why we use 3.3
2 Answers Dr Reddys, IPCA, Reddy Labs,
How you can calculate pKa ?
injection volume accruacy test i calibration of hplc
What is the difference between the working standard and reference standard?
How precise should be the linearity in HPLC method validation?