How to know HPLC column performance?
Answers were Sorted based on User's Feedback
Answer / vijay muley
1) check the back pressure,
2) check Theoretical plates,
3) system suitability as per test requirement e.g. tailing
factor, symmetry, RRT, resolution etc.
all three parameters shows columns performance.
| Is This Answer Correct ? | 20 Yes | 0 No |
Answer / darose
check for the number of plates that it maintains stady all
over the run , check the SST at avery run that cpmplies the
acceptance criterias of the method.
| Is This Answer Correct ? | 5 Yes | 2 No |
Answer / venkat reddy
checked the column ressure
check theoretical plates
asymmentry RRT Resolution tailing factor compound method analysis
| Is This Answer Correct ? | 0 Yes | 0 No |
Answer / deepak
To check theoretical plates,peak shape and resolution means sst of method.
| Is This Answer Correct ? | 0 Yes | 0 No |
Describe your experience with analytical instrumentation?
3 Answers Alembic, Apotex, Aurobindo, DLF, Enal Drugs, Lupin, Natco, Pharma,
The boiling point of 1,3 butadiene is -4.4 DC & the boiling point of Pentane is 36.1 DC. Then how come Pentane elute before 1,3 BD in GC analysis?
why we use actone for Gpv test in hplc calibration
what is the concept for adjustment of pH in HPLC buffer solution ? a. Common ion effect b. Counter ion effect and explain me, how we adapt this principles & what is the role of these principles in our method
1 Answers Apex, Pentagon Labs,
How to fix the sample conc. in Related substances test by HPLC?
One pH means how much volts and milivolts?
how to qualify the impurity?
what is the importence and role of LCMS and LCMS/MS and their applications? which type of compounds are analysis by lcms?
if the flow rate is increases then any changes in retention time in hplc
what is the differnce between GMP and CGMP
WHY POTASSIUM HYDROGEN PTHALATE IS USED FOR PERCHLORIC ACID? What is difference btw UV detector & RID detector? what is dissolution? What is unit of KF? what is the significant of pH in HPlc?
difference between HPLC & LC-MS