Golgappa.net | Golgappa.org | BagIndia.net | BodyIndia.Com | CabIndia.net | CarsBikes.net | CarsBikes.org | CashIndia.net | ConsumerIndia.net | CookingIndia.net | DataIndia.net | DealIndia.net | EmailIndia.net | FirstTablet.com | FirstTourist.com | ForsaleIndia.net | IndiaBody.Com | IndiaCab.net | IndiaCash.net | IndiaModel.net | KidForum.net | OfficeIndia.net | PaysIndia.com | RestaurantIndia.net | RestaurantsIndia.net | SaleForum.net | SellForum.net | SoldIndia.com | StarIndia.net | TomatoCab.com | TomatoCabs.com | TownIndia.com
Interested to Buy Any Domain ? << Click Here >> for more details...

How to know HPLC column performance?

Answer Posted / vijay muley

1) check the back pressure,
2) check Theoretical plates,
3) system suitability as per test requirement e.g. tailing
factor, symmetry, RRT, resolution etc.
all three parameters shows columns performance.

Is This Answer Correct ?    20 Yes 0 No



Post New Answer       View All Answers


Please Help Members By Posting Answers For Below Questions

What is the calibration of uv process and preparation

1993


What is the requirement for brazil in the analytical method vslidation comparr to ich?

757


In BP2013, Loperamid HCl monograph. Assay by titration with 0.1N sodium hydroxide using hydrocloric acid 0.01N and reading the volume added between the 2 points of inflexion. I have a question that if the diluent solvent is ethanol is certainly consumed a amount of volume of titrant, so this volume must be eliminated on the result calculation or not apart from first point which is subtracted above.

3121


WHAT IS THE PARAMETER FOR THE SELECTION OF MOBILE PHASE IN HPLC?

1180


how you fix the limits of impurities?

831


[3/28, 20:52] Manoj P Venkatpurwar: how many impurities require to inject in assay specificity that how we can find out? and in Rs also how?

789


could negative ions be produced by bombardment process in mass spectrometry?

1770


why glutent are detected in the rice cereal baby food product even manufacturer claimed that they are using rice and milk only?we have using ELISA to do the test,and rice supposed not containing any glutent,rite?We already repeat the test so many times and it still detected.just wondering where the glutent came from?

2757


why we use a particular hplc column for a particular compound give reasons?please

2743


What is the formula for relative diffrence for standard solution in solution stability in validation?

787


What if impurity area in control sample coming more as compared to LOQ level of impurity ?

1593


Why do we use KMnO4 in the test of control of obsorbance ? and why do we take specific quantity i.e 57-63mg?

2822


What is related substance by HPLC impurity limits as per USP?

1089


can we use the same detector in HPLC as well GC and what could be the differences we can find in the final chromato graph in any aspects?

2173


why cone formation during dissolution?

774